AGO3 Recombinant Monoclonal Antibody

Code CSB-RA875700A0HU
Size US$210
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Product Details

Uniprot No.
Target Names
AGO3
Alternative Names
5730550L01Rik antibody; Ago 3 antibody; Ago3 antibody; AGO3_HUMAN antibody; argonaute 3 antibody; Argonaute3 antibody; eIF 2C 3 antibody; eIF-2C 3 antibody; eIF2C 3 antibody; Eif2c3 antibody; EIF2C3 protein antibody; Eukaryotic translation initiation factor 2C 3 antibody; Eukaryotic translation initiation factor 2C3 antibody; FLJ12765 antibody; hAgo3 antibody; MGC86946 antibody; Protein argonaute-3 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human AGO3
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
4F7
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The generation of the AGO3 antibody by CUSABIO involves a multi-step process. Initially, an animal is immunized with a synthesized peptide derived from human AGO3. The antibody produced in response to this immunization is then sequenced, and its corresponding antibody gene is synthesized. CUSABIO subsequently clones the AGO3 antibody gene into plasma vectors, which are subsequently introduced into mammalian cells using a lipid-based transfection reagent. Following transient expression, the recombinant antibodies against AGO3 are harvested and thoroughly characterized. The AGO3 recombinant monoclonal antibody is purified using affinity chromatography from the culture medium. It is specifically designed for the detection of human AGO3 protein in ELISA.

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Target Background

Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
Gene References into Functions
  1. Data show that recombinant AGO3 loaded with miR-20a cleaves complementary target RNAs. PMID: 29040713
  2. Study uncovers a first example of a vertebrate protein factor, Argonaute-3, specifically affecting the guide-to-passenger-strand ratio of the miRNA let-7a. A multi-layered mechanism for the observed impact of Ago3 on the let-7a-3p passenger strand expression and activity is proposed. PMID: 24100239
  3. The N-terminal domain of Ago3 inhibits cleavage activity. AAs 1-64 are largely unstructured & form a large loop surrounding an area containing two helices & another unstructured loop. PMID: 23665583
  4. The Ago3 PIWI domain is slicing competent but our Ago3 model suggests multiple interactions between residues in the PIWI & N domains that may increase protein rigidity or invoke other properties that contribute to Ago3's observed slicing deficiency. PMID: 23748378
  5. DICER- and AGO3-dependent generation of retinoic acid-induced DR2 Alu RNAs regulates human stem cell proliferation. PMID: 23064648
  6. Ago3 is able to load microRNAs efficiently in the absence of Ago1 and Ago2, despite a significant loss of global microRNA expression PMID: 22474261
  7. reliable predictions of miRNA affinity to Ago2 AND Ago3 proteins were made. PMID: 21634124
  8. The specificity of RNA interference depends on the concentration of Ago1, Ago3, and Ago4 relative to Ago2. PMID: 18771919
  9. EIF@C3 protein is expressed in both Schwann and neuron-type differentiating cells. PMID: 19393748

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Subcellular Location
Cytoplasm, P-body.
Protein Families
Argonaute family, Ago subfamily
Database Links

HGNC: 18421

OMIM: 607355

KEGG: hsa:192669

STRING: 9606.ENSP00000362287

UniGene: Hs.657659

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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