AGPAT3 Antibody

Code CSB-PA865125LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: K562 whole cell lysate
    All lanes: AGPAT3 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 44, 37, 46 kDa
    Observed band size: 44 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA865125LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA865125LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) AGPAT3 Polyclonal antibody
Uniprot No.
Target Names
AGPAT3
Alternative Names
AGPAT3; LPAAT3; UNQ759/PRO1490; 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma; 1-acylglycerol-3-phosphate O-acyltransferase 3; 1-AGP acyltransferase 3; 1-AGPAT 3; Lysophosphatidic acid acyltransferase gamma; LPAAT-gamma
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma protein (148-304AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The AGPAT3 Antibody (Product code: CSB-PA865125LA01HU) is Non-conjugated. For AGPAT3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA865125LB01HU AGPAT3 Antibody, HRP conjugated ELISA
FITC CSB-PA865125LC01HU AGPAT3 Antibody, FITC conjugated
Biotin CSB-PA865125LD01HU AGPAT3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor. Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA. Has a preference for arachidonoyl-CoA as a donor. Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol.
Gene References into Functions
  1. The data is consistent with a structural arrangement in which motif I is located in the cytoplasm and motif II is in the endoplasmic reticulum and Golgi lumen, suggesting a different model for AGPAT3/LPAAT3's enzymatic mechanism. PMID: 20537980
  2. Of the two well conserved acyltransferase motifs, NHX(4)D is present in AGPAT8, whereas arginine in the EGTR motif is substituted by aspartate. PMID: 16620771
  3. Results report the identification of an integral membrane lysophosphatidic acid-specific acyltransferase, LPAAT3, which regulates Golgi membrane tubule formation, trafficking, and structure by altering phospholipids and lysophospholipids. PMID: 19635840
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus envelope.
Protein Families
1-acyl-sn-glycerol-3-phosphate acyltransferase family
Tissue Specificity
Widely expressed with highest levels in testis, pancreas and kidney, followed by spleen, lung, adipose tissue and liver.
Database Links

HGNC: 326

OMIM: 614794

KEGG: hsa:56894

STRING: 9606.ENSP00000291572

UniGene: Hs.248785

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