AGPS Antibody

Datasheet

Code	CSB-PA001457LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: AGPS antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa IHC image of CSB-PA001457LA01HU diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of NIH/3T3 cells with CSB-PA001457LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AGPS Polyclonal antibody

Uniprot No.

O00116

Target Names

AGPS

Alternative Names

AAG5 antibody; ADAP-S antibody; ADAS antibody; ADAS_HUMAN antibody; ADHAPS antibody; ADPS antibody; Aging associated gene 5 protein antibody; Aging-associated gene 5 protein antibody; aging-associated protein 5 antibody; AGPS antibody; ALDHPSY antibody; Alkyl-DHAP synthase antibody; Alkyldihydroxyacetonephosphate synthase antibody; Alkyldihydroxyacetonephosphate synthase, peroxisomal antibody; Alkylglycerone phosphate synthase antibody; Alkylglycerone-phosphate synthase antibody; peroxisomal antibody; RCDP3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Alkyldihydroxyacetonephosphate synthase, peroxisomal protein (279-400AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The AGPS Antibody (Product code: CSB-PA001457LA01HU) is Non-conjugated. For AGPS Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001457LB01HU	AGPS Antibody, HRP conjugated	ELISA
FITC	CSB-PA001457LC01HU	AGPS Antibody, FITC conjugated
Biotin	CSB-PA001457LD01HU	AGPS Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the exchange of the acyl chain in acyl-dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding the first ether linked intermediate, i.e. alkyl-dihydroxyacetonephosphate (alkyl-DHAP), in the pathway of ether lipid biosynthesis.

Gene References into Functions

we optimized the BITC construction targeting alkylglycerone phosphate synthase (AGPS) by computer-aided design, and the derivants also showed anti-tumor potential in vitro. PMID: 25542233
AGPS overexpression could be a causation of chemotherapy agent resistance of cancer cells. AGPS silencing could lead to drug uptake and cell cycle arrest and apoptotic cell death. PMID: 24815474
Results demonstrated that AGPS negatively regulated the invasion potential of glioma and hepatic carcinoma cells by modulating the expression of relevant genes and activity of the MAPK pathway. PMID: 24841318
AGPS, in addition to maintaining ether lipids, also controls cellular utilization of fatty acids, favoring the generation of signaling lipids necessary for promoting the aggressive features of cancer. PMID: 23980144
Novel mutations in AGPS (alkylglycerone-phosphate synthase) cause rhizomelic chondrodysplasia punctata (RCDP) type 3. PMID: 21990100
Defect of ADAPS expression was also assessed by immunoblot PMID: 18571506

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Involvement in disease

Rhizomelic chondrodysplasia punctata 3 (RCDP3)

Subcellular Location

Peroxisome membrane. Peroxisome.

Protein Families

FAD-binding oxidoreductase/transferase type 4 family

Database Links

HGNC: 327

OMIM: 600121

KEGG: hsa:8540

STRING: 9606.ENSP00000264167

UniGene: Hs.516543

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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