AHI1 Antibody

Datasheet
Code CSB-PA836642LA01HU
See More Details Free Antibody trial simple
Size US$299
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate, U251 whole cell lysate
    All lanes: AHI1 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 138, 121, 71 kDa
    Observed band size: 138 kDa

  • IHC image of CSB-PA836642LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AHI1 Polyclonal antibody
Uniprot No. Q8N157
Target Names AHI1
Alternative Names Jouberin (Abelson helper integration site 1 protein homolog) (AHI-1), AHI1
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Jouberin protein (1-213AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Involved in vesicle trafficking and required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in neuronal differentiation.
Involvement in disease Joubert syndrome 3 (JBTS3)
Subcellular Location Cytoplasm, cytoskeleton, cilium basal body, Cell junction, adherens junction, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Tissue Specificity Highly expressed in the most primitive normal hematopoietic cells. Expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Expressed in kidney (renal collecting duct ce
Database Links

HGNC: 21575

OMIM: 608629

KEGG: hsa:54806

STRING: 9606.ENSP00000265602

UniGene: Hs.386684

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