AOX1 Antibody

Code CSB-PA001858HA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA001858HA01HU at dilution of 1:100

  • Immunofluorescence staining of HepG2 cells with CSB-PA001858HA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) AOX1 Polyclonal antibody
Uniprot No.
Target Names
AOX1
Alternative Names
AOX1 antibody; AOAldehyde oxidase antibody; EC 1.2.3.1 antibody; Aldehyde oxidase 1 antibody; Azaheterocycle hydroxylase antibody; EC 1.17.3.- antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Aldehyde oxidase protein (236-421AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The AOX1 Antibody (Product code: CSB-PA001858HA01HU) is Non-conjugated. For AOX1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA001858HB01HU AOX1 Antibody, HRP conjugated ELISA
FITC CSB-PA001858HC01HU AOX1 Antibody, FITC conjugated
Biotin CSB-PA001858HD01HU AOX1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.
Gene References into Functions
  1. AOX1 SNPs exist in which the base-pair exchange of the SNP leads to loss of enzyme activity. PMID: 26842593
  2. ten novel single nucleotide polymorphisms resulting in amino acid exchanges in proximity to the FAD site of hAOX1, were characterized. PMID: 28750088
  3. Data suggest that substrate specificity of AOX1 includes GDC-0834, an inhibitor of Bruton's tyrosine kinase with potential non-steroidal anti-inflammatory agent activity; in liver cytosol, hydrolysis of GDC-0834 is primarily due to AOX1. PMID: 25845827
  4. Methotrexate is poorly metabolized by human AOX1, in contrast to rabbit. PMID: 26032640
  5. These results supported the possibility that XO/XD and AO can contribute to nitric oxide generation. PMID: 25537183
  6. Several donors have a normal AOX1 protein level. PMID: 23857892
  7. High AOX1 methylation was associated with biochemical recurrence in prostate cancer. PMID: 23918943
  8. The physiological role of AOX1 remains unclear, but it is known that thiopurines belong to its substrates; AOX1 contributes to azathioprine catabolism. PMID: 22495427
  9. hydralazine, at a concentration of 25 to 50 muM, can be used in human hepatocyte incubations to estimate the contribution of aldehyde oxidase to the hepatic clearance of drugs and other compounds. PMID: 22522748
  10. Human population is characterized by the presence of functionally inactive hAOX1 allelic variants as well as variants encoding enzymes with different catalytic activities. PMID: 22279051
  11. characterization of AO activity in cryopreserved human hepatocytes: enzyme stability; substrate specificity; kinetics; inhibition by hydralazine PMID: 22031625
  12. Expressed AOX1 in Escherichia coli and purified kinetically active protein. PMID: 19741035

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Subcellular Location
Cytoplasm.
Protein Families
Xanthine dehydrogenase family
Tissue Specificity
Abundant in liver, expressed in adipose tissue and at lower levels in lung, skeletal muscle, pancreas. In contrast to mice, no significant gender difference in AOX1 expression level (at protein level).
Database Links

HGNC: 553

OMIM: 602841

KEGG: hsa:316

STRING: 9606.ENSP00000363832

UniGene: Hs.406238

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