AP2A2 Antibody

Code CSB-PA001870DSR2HU
Size US$166
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  • Western blot
    All lanes: AP2A2 antibody at 0.61μg/ml + Mouse brain tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 104, 105, 74 kDa
    Observed band size: 104 kDa

  • Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA001870DSR2HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA001870DSR2HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) AP2A2 Polyclonal antibody
Uniprot No.
Target Names
AP2A2
Alternative Names
100 kDa coated vesicle protein C antibody; Adaptor protein complex AP-2 subunit alpha-2 antibody; Adaptor-related protein complex 2 subunit alpha-2 antibody; Alpha-adaptin C antibody; Alpha2-adaptin antibody; AP-2 complex subunit alpha-2 antibody; Ap2a2 antibody; AP2A2_HUMAN antibody; Clathrin assembly protein complex 2 alpha-C large chain antibody; HIP-9 antibody; Huntingtin yeast partner J antibody; Huntingtin-interacting protein 9 antibody; Huntingtin-interacting protein J antibody; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human AP-2 complex subunit alpha-2 protein (590-939AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
Gene References into Functions
  1. AP2 has evolved as a key regulatory node to coordinate clarhtin-coated pit formation and cargo sorting and ensure high spatial and temporal regulation of cathrin-mediated endocytosis. PMID: 28003333
  2. the AP-2 complex is not associated with the endocytosed plasma membrane-associated clathrin PMID: 14530274
  3. The mutation of lysine-297 and arginine-340 on the alpha-adaptin specifically inhibits the ability of Nef to bind AP-2 and downregulate CD4. PMID: 19129443
  4. Clathrin-dependent endocytosis of human CD317 from the cell surface lipid rafts is mediated by direct interaction with alpha-adaptin. PMID: 19359243
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Adaptor complexes large subunit family
Tissue Specificity
Expressed in the brain (at protein level).
Database Links

HGNC: 562

OMIM: 607242

KEGG: hsa:161

STRING: 9606.ENSP00000413234

UniGene: Hs.19121

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