APCDD1 Antibody

Datasheet

Code	CSB-PA814246LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate All lanes: APCDD1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa IHC image of CSB-PA814246LA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA814246LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) APCDD1 Polyclonal antibody

Uniprot No.

Q8J025

Target Names

APCDD1

Alternative Names

Adenomatosis polyposis coli down regulated 1 antibody; Adenomatosis polyposis coli down regulated 1 protein antibody; Adenomatosis polyposis coli down-regulated 1 protein antibody; APCD1_HUMAN antibody; APCDD 1 antibody; APCDD1 antibody; B7323 antibody; DRAPC1 antibody; FP7019 antibody; HHS antibody; HTS antibody; hypoptrichosis simplex antibody; Protein APCDD1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein APCDD1 protein (22-288AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The APCDD1 Antibody (Product code: CSB-PA814246LA01HU) is Non-conjugated. For APCDD1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA814246LB01HU	APCDD1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA814246LC01HU	APCDD1 Antibody, FITC conjugated
Biotin	CSB-PA814246LD01HU	APCDD1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Negative regulator of the Wnt signaling pathway. Inhibits Wnt signaling in a cell-autonomous manner and functions upstream of beta-catenin. May act via its interaction with Wnt and LRP proteins. May play a role in colorectal tumorigenesis.

Gene References into Functions

Thus, we have provided the first evidence that APCDD1 expression is epigenetically silenced in OS, which may facilitate invasion and metastasis of OS cells. PMID: 28698141
these novel findings suggest that APCDD1 positively regulates adipogenic differentiation and that its down-regulation by miR-130 during diet-induced obesity may contribute to impaired adipogenic differentiation and obesity-related metabolic disease. PMID: 28242765
This study demonstrated a critical role for Apcdd1 in OL differentiation after white matter injury that points to a potential therapeutic approach for inhibiting Wnt signaling in these disorders. PMID: 25946682
Unusual role of APCDD1 in dental follicle cells during osteogenic differentiation. APCDD1 sustains the expression and activation of beta-catenin. PMID: 25592970
mutation in the APCDD1 gene is responsible for hereditary hypotrichosis simplex in a large Chinese family. PMID: 22512811
Data show that the methylated VAPA-APCDD1 DNA in maternal plasma is predominantly derived from the fetus, and this novel fetal epigenetic marker in maternal plasma is useful for the noninvasive detection of fetal trisomy 18. PMID: 21152411
APCDD1 is a novel inhibitor of the Wnt signalling pathway with an essential role in human hair growth PMID: 20393562

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Involvement in disease

Hypotrichosis 1 (HYPT1)

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

APCDD1 family

Tissue Specificity

Abundantly expressed in heart, pancreas, prostate and ovary. Moderately expressed in lung, liver, kidney, spleen, thymus, colon and peripheral lymphocytes. Abundantly expressed in both the epidermal and dermal compartments of the hair follicle. Present in

Database Links

HGNC: 15718

OMIM: 605389

KEGG: hsa:147495

STRING: 9606.ENSP00000347433

UniGene: Hs.293274

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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