ATG4B Antibody, HRP conjugated

Code CSB-PA002290LB01HU
Size US$166
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ATG4B Polyclonal antibody
Uniprot No.
Target Names
ATG4B
Alternative Names
APG4 autophagy 4 homolog B antibody; APG4B antibody; ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody; ATG4B antibody; ATG4B_HUMAN antibody; AUT like 1 cysteine endopeptidase antibody; AUT-like 1 cysteine endopeptidase antibody; AUTL1 antibody; Autophagin 1 antibody; Autophagin-1 antibody; Autophagy related 4B cysteine peptidase antibody; Autophagy related cysteine endopeptidase 1 antibody; Autophagy related protein 4 homolog B antibody; Autophagy-related cysteine endopeptidase 1 antibody; Autophagy-related protein 4 homolog B antibody; Cysteine protease ATG4B antibody; hAPG4B antibody; MGC1353 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Cysteine protease ATG4B protein (350-400AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
HRP
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Gene References into Functions
  1. ATG4B expression was observed markedly upregulated by EWS-FLI1 overexpression, and silencing of ATG4B dramatically inhibits autophagy in Ewing sarcoma cells. PMID: 28902354
  2. study demonstrates that Rab7b regulates LC3 processing by modulating Atg4B activity; taken together, findings reveal Rab7b as a novel negative regulator of autophagy through its interaction with Atg4B PMID: 28835545
  3. ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8 PMID: 28287329
  4. O-GlcNAcylation of ATG4B regulates autophagy activation by increasing its proteolytic activity under metabolic stress condition PMID: 27527864
  5. these results demonstrate a novel association of ATG4B positive expression with HER2 positive breast cancers PMID: 27556700
  6. Knockdown of ATG4B had only a minor effect on AMPK activation and G1 phase arrest in liver kinase B1-deficient or AMPK-inhibited cancer cells. PMID: 29169176
  7. Our work describes an MST4-ATG4B signaling axis that influences glioblastoma autophagy and malignancy PMID: 29232556
  8. Data show that ULK1, a protein kinase activated at the autophagosome formation site, phosphorylates human ATG4B on serine 316. PMID: 28821708
  9. Reporter assay showed that HSF1 increased the transcriptional activity of ATG4B gene promoter, and chromatin immunoprecipitation assay verified that HSF1 bound to the site (-1429 to -1417) in ATG4B gene promoter region. PMID: 28889000
  10. The structure and regulatory machinery of Atg4b [review] PMID: 28253956
  11. MIRlet-7i is able to regulate autophagic activity via regulating Atg4B expression, which might contribute to the pathogenesis of pre-eclampsia. PMID: 27770612
  12. Results demonstrated that upregulation of miR-34a by transfection or demethylation resulted in the enhanced apoptosis and drug sensitivity in prostate cancer cells. ATG4B, directly regulated by miR-34a through AMPK/mTOR, was involved in this process. PMID: 26499184
  13. Purified ATG4B protein interact with LC3B in vitro. PMID: 25652856
  14. ATG4B independently plays a role as a positive regulator on tumor proliferation and a negative regulator on autophagy in colorectal cancer cells. PMID: 24991826
  15. This study identifies ATG4B as a potential biomarker for predicting therapeutic response in treatment-naive chronic myeloid leukemia stem/progenitor cells and uncovers ATG4B as a possible drug target in these cells. PMID: 24755409
  16. The actions of ATG4 family members (particularly ATG4B) are required for the control of autophagosome fusion with late, degradative compartments in differentiating human erythroblasts. PMID: 23508006
  17. HsAtg4B negatively regulates the localization of LC3 to a membrane compartment by delipidation PMID: 15187094
  18. analysis of specificity and catalysis of HsAtg4B PMID: 16183633
  19. crystal structure of human Atg4b by X-ray crystallography at 2.0 A resolution, and show that Atg4b is a cysteine protease whose active catalytic triad site consists of Cys74, His280 and Asp278 PMID: 16325851
  20. Murine Atg8L/Apg8L modification is mediated by human Atg4B. PMID: 16704426
  21. The crystal structures of catalytically inert human Atg4B in complex with processed and unprocessed forms of LC3 showed that, on LC3 binding, the regulatory loop and the N-terminal tail of HsAtg4B undergo large conformational changes. PMID: 19322194

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Subcellular Location
Cytoplasm.
Protein Families
Peptidase C54 family
Database Links

HGNC: 20790

OMIM: 611338

KEGG: hsa:23192

STRING: 9606.ENSP00000384259

UniGene: Hs.283610

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