ATP5F1D Antibody

Datasheet

Code	CSB-PA00635A0Rb
Size	US$166
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Image	Western blot All lanes: ATP5F1D antibody at 2µg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA00635A0Rb at dilution of 1:100 Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00635A0Rb at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP5F1D Polyclonal antibody

Uniprot No.

P30049

Target Names

ATP5F1D

Alternative Names

ATP synthase subunit delta; mitochondrial antibody; ATP synthase subunit delta; mitochondrial antibody; ATP synthase; H+ transporting; mitochondrial F1 complex; delta subunit antibody; ATP5D antibody; ATPD_HUMAN antibody; F ATPase delta subunit antibody; F-ATPase delta subunit antibody; Mitochondrial ATP synthase complex delta subunit precusor antibody; Mitochondrial ATP synthase delta subunit antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human ATP synthase subunit delta, mitochondrial protein (32-158AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP5F1D Antibody (Product code: CSB-PA00635A0Rb) is Non-conjugated. For ATP5F1D Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA00635B0Rb	ATP5F1D Antibody, HRP conjugated	ELISA
FITC	CSB-PA00635C0Rb	ATP5F1D Antibody, FITC conjugated
Biotin	CSB-PA00635D0Rb	ATP5F1D Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene References into Functions

These data establish c.245C>T (p.Pro82Leu) and c.317T>G (p.Val106Gly) in ATP5F1D as pathogenic variants leading to a Mendelian mitochondrial disease featuring episodic metabolic decompensation. PMID: 29478781
Immunohistochemical and immunofluorescent double staining of ATP5D and synaptophysin showed that the reduction of ATP5D was most pronounced at synapses in amyotrophic lateral sclerosis PMID: 27899032

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

Protein Families

ATPase epsilon chain family

Database Links

HGNC: 837

OMIM: 603150

KEGG: hsa:513

STRING: 9606.ENSP00000215375

UniGene: Hs.418668

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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