ATP5F1D Antibody

Datasheet
Code CSB-PA002355HA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA002355HA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA002355HA01HU at dilution of 1:100

  • Immunofluorescence staining of A549 cells with CSB-PA002355HA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) ATP5F1D Polyclonal antibody
Uniprot No. P30049
Target Names ATP5F1D
Alternative Names ATP synthase subunit delta; mitochondrial antibody; ATP synthase subunit delta; mitochondrial antibody; ATP synthase; H+ transporting; mitochondrial F1 complex; delta subunit antibody; ATP5D antibody; ATPD_HUMAN antibody; F ATPase delta subunit antibody; F-ATPase delta subunit antibody; Mitochondrial ATP synthase complex delta subunit precusor antibody; Mitochondrial ATP synthase delta subunit antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human ATP synthase subunit delta, mitochondrial protein (23-168AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Gene References into Functions
  1. These data establish c.245C>T (p.Pro82Leu) and c.317T>G (p.Val106Gly) in ATP5F1D as pathogenic variants leading to a Mendelian mitochondrial disease featuring episodic metabolic decompensation. PMID: 29478781
  2. Immunohistochemical and immunofluorescent double staining of ATP5D and synaptophysin showed that the reduction of ATP5D was most pronounced at synapses in amyotrophic lateral sclerosis PMID: 27899032
Subcellular Location Mitochondrion, Mitochondrion inner membrane
Protein Families ATPase epsilon chain family
Database Links

HGNC: 837

OMIM: 603150

KEGG: hsa:513

STRING: 9606.ENSP00000215375

UniGene: Hs.418668

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