ATP5F1 Antibody

Datasheet

Code	CSB-PA002358ESR1HU
Size	US$166
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Image	Western blot All lanes: ATP synthase F (0) complex subunit B1, mitochondrial antibody at 4μg/ml Lane 1: Mouse heart tissue Lane 2: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA002358ESR1HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP5F1 Polyclonal antibody

Uniprot No.

P24539

Target Names

ATP5F1

Alternative Names

AT5F1_HUMAN antibody; ATP synthase B chain mitochondrial antibody; ATP synthase subunit b antibody; ATP synthase subunit b mitochondrial antibody; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit b; antibody; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit b; isoform 1 antibody; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit B1 antibody; ATP5F1 antibody; ATPase subunit b antibody; Cell proliferation inducing protein 47 antibody; H+ ATP synthase subunit b antibody; MGC24431 antibody; mitochondrial antibody; PIG47 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human ATP synthase F(0) complex subunit B1, mitochondrial protein (1-245AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Gene References into Functions

Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance. PMID: 20038517
Nitration of tyrosine residues 368 and 345 in the beta-subunit elicits FoF1-ATPase activity loss. PMID: 19650768

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

Protein Families

Eukaryotic ATPase B chain family

Database Links

HGNC: 840

OMIM: 603270

KEGG: hsa:515

STRING: 9606.ENSP00000358737

UniGene: Hs.514870

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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