ATP6V1G1 Antibody

Datasheet

Code	CSB-PA00135A0Rb
Size	US$166
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Image	Western blot All lanes: V-type proton ATPase subunit G 1 antibody at 2μg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00135A0Rb at dilution of 1:50 Immunofluorescence staining of PC-3 cells with CSB-PA00135A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00135A0Rb at dilution of 1:100
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Product Details
Citations
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP6V1G1 Polyclonal antibody

Uniprot No.

O75348

Target Names

ATP6V1G1

Alternative Names

ATP 6G antibody; ATP 6J antibody; ATP6G antibody; ATP6G1 antibody; ATP6GL antibody; Atp6v1g1 antibody; ATPase H+ transporting lysosomal (vacuolar proton pump) member J antibody; ATPase H+ transporting lysosomal 13kD V1 subunit G isoform 1 antibody; ATPase H+ transporting lysosomal 13kDa V1 subunit G1 antibody; ATPase H+ transporting lysosomal member J antibody; ATPase H+ transporting lysosomal subunit G isoform 1 antibody; DKFZp547P234 antibody; V ATPase 13 kDa subunit 1 antibody; V ATPase G subunit 1 antibody; V-ATPase 13 kDa subunit 1 antibody; V-ATPase subunit G 1 antibody; V-type proton ATPase subunit G 1 antibody; Vacuolar ATP synthase subunit G 1 antibody; Vacuolar ATP synthase subunit G1 antibody; Vacuolar ATP synthase subunit M16 antibody; Vacuolar H+ ATPase G1 antibody; Vacuolar H+ ATPase subunit G 1 antibody; Vacuolar proton pump G subunit 1 antibody; Vacuolar proton pump subunit G 1 antibody; Vacuolar proton pump subunit M16 antibody; VATG1_HUMAN antibody; Vma 10 antibody; Vma10 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human V-type proton ATPase subunit G 1 protein (2-118AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP6V1G1 Antibody (Product code: CSB-PA00135A0Rb) is Non-conjugated. For ATP6V1G1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA00135B0Rb	ATP6V1G1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA00135C0Rb	ATP6V1G1 Antibody, FITC conjugated
Biotin	CSB-PA00135D0Rb	ATP6V1G1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Molecular Mechanism of Renal Tubule Transport H ϩ-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney Frische S, et al,American Journal of Physiology-Renal Physiology,2019
The H+-ATPase B1 subunit localizes to the thick ascending limb and distal convoluted tubule of rodent and human kidney Sebastian Frische.et al,American Journal of Physiology-Renal Physiology,2018

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 2 reviews

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Applications : IF/IHC

Sample type: Mouse, human Mouse and human kidney tissue

Review: H+-ATPase G1 subunit is expressed along the mouse and human distal nephron.

By Anonymous

Applications : Immunostaining

Sample type: Mouse Mouse kidney

Review: Immunostaining for H + -ATPase G1 subunit (ATP6V1G1) in mouse kidney showed expression in the cortical and medullary portion of the kidney. Staining was found in both the proximal tubules and distal nephron segments, with pronounced intercalated cell (IC) staining in both cortex and medulla. Gray stippled lines demarcate the zone of the inner medulla.

By Anonymous

Target Background

Function

Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.

Gene References into Functions

RILP regulates vacuolar ATPase through interaction with the V1G1 subunit PMID: 26180254
genome-wide association study in population in China: Data suggest 4 SNPs upstream of ATP6V1G1 (rs10817638, rs7851259, rs10982287, rs4979427) are associated (in bivariate manner) with age at menarche and bone density. [includes META-ANALYSIS] PMID: 26312577
RILP regulates the activity of the V-ATPase through its interaction with V1G1. PMID: 24762812

Subcellular Location

Apical cell membrane.

Protein Families

V-ATPase G subunit family

Tissue Specificity

Kidney; localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). Ubiquitous.

Database Links

HGNC: 864

OMIM: 607296

KEGG: hsa:9550

STRING: 9606.ENSP00000363162

UniGene: Hs.388654

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

mTOR signaling pathway Oxidative Phosphorylation Synaptic vesicle cycle

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