Acetyl-HIST1H1C (K96) Antibody

Datasheet
Code CSB-PA010378PA96acHU
Size US$319
Uniprot No. P16403
Image
  • Western Blot
    Detected sample: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
    All lanes: HIST1H1C antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunocytochemistry analysis of Hela cells using CSB-PA010378PA96acHU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) tissue using CSB-PA010378PA96acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8μg anti-HIST1H1c (CSB-PA010378PA96acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • Immunoprecipitating HIST1H1C in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h)
    Lane 1: Rabbit control IgG instead of CSB-PA010378PA96acHU in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA010378PA96acHU (5μg) + Hela whole cell lysate (treated with 30mM sodium butyrate for 4h)500μg)
    Lane 3: Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (20μg)

Immunogen Peptide sequence around site of Acetyl-Lys (96) derived from Human Histone H1.2.
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, ICC, IF, IP, ChIP; Recommended dilution: WB:1:1000-1:5000, ICC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Relevance Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H1C
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4716

OMIM: 142710

KEGG: hsa:3006

STRING: 9606.ENSP00000339566

UniGene: Hs.7644

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