Acetyl-HIST1H1C (K96) Antibody

Western Blot
Detected sample: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H1C antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

Immunocytochemistry analysis of Hela cells using CSB-PA010378PA96acHU at dilution of 1:100

Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) tissue using CSB-PA010378PA96acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Chromatin Immunoprecipitation Hela (4*10⁶, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8μg anti-HIST1H1c (CSB-PA010378PA96acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Immunoprecipitating HIST1H1C in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010378PA96acHU in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010378PA96acHU (5μg) + Hela whole cell lysate (treated with 30mM sodium butyrate for 4h)500μg)
Lane 3: Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (20μg)