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Acetyl-HIST1H1C (K96) Antibody

Datasheet

Code	CSB-PA010378PA96acHU
Size	US$319
Image	Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H1C antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa Immunocytochemistry analysis of Hela cells using CSB-PA010378PA96acHU at dilution of 1:100 Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010378PA96acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Chromatin Immunoprecipitation Hela (4*10⁶, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1C (CSB-PA010378PA96acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter. Immunoprecipitating HIST1H1C in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) Lane 1: Rabbit control IgG instead of CSB-PA010378PA96acHU in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010378PA96acHU (5µg) + Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg) Lane 3: Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
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Product Details
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Target Data

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HIST1H1C Polyclonal antibody

Uniprot No.

P16403

Target Names

HIST1H1C

Alternative Names

H1 histone family member 2 antibody; H1.a antibody; H12_HUMAN antibody; H1F2 antibody; H1s-1 antibody; HIST1H1C antibody; Histone 1 H1c antibody; Histone cluster 1 H1c antibody; Histone H1.2 antibody; Histone H1c antibody; Histone H1d antibody; Histone H1s-1 antibody; MGC3992 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence around site of Acetyl-Lys (96) derived from Human Histone H1.2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, ICC, IF, IP, ChIP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
ICC	1:20-1:200
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Gene References into Functions	results define a network of E2F target genes as susceptible to the regulatory influence of H1.2, where H1.2 augments global association of pRb with chromatin, enhances transcriptional repression by pRb, and facilitates pRb-dependent cell-cycle arrest PMID: 28614707 BRG1 participates in gene repression by interacting with H1.2, facilitating its deposition and stabilizing nucleosome positioning around the transcription start site. PMID: 27390128 Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. Also, the study reports the observation of the H1.2 SNP variant A18V in MCF-10A cells. PMID: 26209608 Integration with apoptotic intermediates (via C-terminal tail interactions) may constitute a more generalized function of linker histone isoforms in apoptotic cascades. PMID: 24525734 Histone H1.2-T165 post translational modifications are dispensable for chromatin binding and cell proliferation while the H1.4-K26 modifications are essential for proper cell cycle progression. PMID: 24873882 H1.2 interacts with Cul4A and PAF1 to activate developmental regulatory genes. PMID: 24360965 H1.2 is less abundant than other histone H1 variants at the transcription start sites of inactive genes, and promoters enriched in H1.2 are different from those enriched in other histone H1 variants and tend to be repressed. PMID: 24476918 Mutations in linker histone genes HIST1H1 B, C, D, and E; OCT2 (POU2F2); IRF8; and ARID1A underlying the pathogenesis of follicular lymphoma. PMID: 24435047 These data suggest that p53 acetylation-H1.2 phosphorylation cascade serves as a unique mechanism for triggering p53-dependent DNA damage response pathways. PMID: 22249259 confirmed N-terminal acetylation on all isoforms plus a single internal acetylation site; phosphorylation sites were located on peptides containing the cyclin dependent kinase (CDK) consensus motif PMID: 15595731 The binding of histone H1 to a general amyloid-like motif indicates that histone H1 may play an important common role in diseases associated with amyloid-like fibrils. PMID: 16854430 Histone H1.2 was translocated from the nucleus to the mitochondria after treatment with bleomycin and co-localized with Bak in mitochondria. PMID: 17879944 that the recruitment of YB1, PURalpha, and H1.2 to the p53 target gene Bax is required for repression of p53-induced transcription. PMID: 18258596 Show More Hide All
Subcellular Location	Nucleus, Chromosome
Protein Families	Histone H1/H5 family
Database Links	HGNC: 4716 OMIM: 142710 KEGG: hsa:3006 STRING: 9606.ENSP00000339566 UniGene: Hs.7644

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