BROX Antibody

Datasheet
Code CSB-PA730136LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q5VW32
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate
    All lanes: BROX antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 47, 43 kDa
    Observed band size: 47 kDa

  • IHC image of CSB-PA730136LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA730136LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human BRO1 domain-containing protein BROX protein (288-409AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Relevance extracellular exosome
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias BRO1 domain-containing protein BROX (BRO1 domain- and CAAX motif-containing protein), BROX, BROFTI C1orf58
Immunogen Species Human
Research Area Cell Biology
Target Names BROX
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Membrane, Lipid-anchor
Protein Families BROX family
Database Links

HGNC: 26512

KEGG: hsa:148362

STRING: 9606.ENSP00000343742

UniGene: Hs.534965

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