BROX Antibody

Code CSB-PA730136LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate
    All lanes: BROX antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 47, 43 kDa
    Observed band size: 47 kDa

  • IHC image of CSB-PA730136LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA730136LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) BROX Polyclonal antibody
Uniprot No.
Target Names
BROX
Alternative Names
BRO1 domain and CAAX motif containing antibody; BRO1 domain containing protein antibody; BRO1 domain- and CAAX motif-containing protein antibody; BRO1 domain-containing protein BROX antibody; BROFTI antibody; Brox antibody; BROX_HUMAN antibody; C1orf58 antibody; FLJ32421 antibody; hCG_25336 antibody; RP11-452F19.1 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human BRO1 domain-containing protein BROX protein (288-409AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The BROX Antibody (Product code: CSB-PA730136LA01HU) is Non-conjugated. For BROX Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA730136LB01HU BROX Antibody, HRP conjugated ELISA
FITC CSB-PA730136LC01HU BROX Antibody, FITC conjugated
Biotin CSB-PA730136LD01HU BROX Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Gene References into Functions
  1. SGK1.1 regulates gene transcription upon a different set of genes some of which participate in cell survival pathways (BAG-4) and others in intracellular vesicular traffic (Brox). PMID: 25849655
  2. structural analysis of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding PMID: 22162750
  3. Results revealed that Strep-Brox(WT) exhibited accumulation in the perinuclear area suggested physiological significance of farnesylation of Brox in its subcellular distribution and efficient interaction with CHMP4s in vivo. PMID: 18190528
Subcellular Location
Membrane; Lipid-anchor.
Protein Families
BROX family
Database Links

HGNC: 26512

KEGG: hsa:148362

STRING: 9606.ENSP00000343742

UniGene: Hs.534965

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