CENPA (Ab-7) Antibody

Datasheet
Code CSB-PA005205OA07nphHU
Size US$319
Uniprot No. P49450
Image
  • IHC image of CSB-PA005205OA07nphHU diluted at 1:10 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA005205OA07nphHU diluted at 1:10 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen Peptide sequence around site of Ser (7) derived from Human Histone H3-like centromeric protein A
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC; Recommended dilution: IHC:1:10-1:100
Relevance Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H3-like centromeric protein A (Centromere autoantigen A) (Centromere protein A) (CENP-A), CENPA
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names CENPA
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes
Subcellular Location Nucleus, Chromosome, centromere, kinetochore, Chromosome, centromere
Protein Families Histone H3 family
Database Links

HGNC: 1851

OMIM: 117139

KEGG: hsa:1058

STRING: 9606.ENSP00000336868

UniGene: Hs.1594

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