CH25H Antibody

Datasheet

Code	CSB-PA005307LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA005307LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005307LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CH25H Polyclonal antibody

Uniprot No.

O95992

Target Names

CH25H

Alternative Names

CH25H; Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase; h25OH

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cholesterol 25-hydroxylase protein (142-272AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CH25H Antibody (Product code: CSB-PA005307LA01HU) is Non-conjugated. For CH25H Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005307LB01HU	CH25H Antibody, HRP conjugated	ELISA
FITC	CSB-PA005307LC01HU	CH25H Antibody, FITC conjugated
Biotin	CSB-PA005307LD01HU	CH25H Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CUSABIO uses the recombinant human CH25H protein (142-272AA) to immunize rabbits and then purify the rabbit antiserum through protein G to obtain the anti-CH25H antibody. This CH25H antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95%. It only reacts with human CH25H protein, an IFN-induced enzyme that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC) to reduce cholesterol accumulation. 25HC, a natural oxysterol, plays a role in regulating cholesterol homeostasis and sterol biosynthesis and exerts broad-spectrum antiviral function. And this anti-CH25H antibody can be used in ELISA and IHC for the detection of the CH25H protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. As an interferon-stimulated gene, has broad antiviral activities against a wide range of enveloped viruses, such as vesicular stomatitis virus (VSV) and SARS coronavirus-2 (SARS-CoV-2). Its product, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication. In testis, production of 25-hydroxycholesterol by macrophages plays a role in Leydig cell differentiation.

Gene References into Functions

We examined whether 25HC restricts infection by mammalian reovirus. Results suggest that 25HC inhibits the efficiency of cellular entry of reovirus virions, which may require specific endosomal membrane dynamics for efficient membrane penetration. PMID: 29950420
We detected Cholesterol 25-hydroxylase, the enzyme responsible for 25-HC production, in human spermatozoa at the level of the neck and the post acrosomal area. Upon incubation with spermatozoa, 25-HC induced calcium and cholesterol transients in connection with the acrosomal reaction. PMID: 27912195
Exome analysis in 212 multiple sclerosis and 14 neuromyelitis optica patients identified a rare CH25H p.Q17H mutation in two NMO patients of Asian ancestry. In addition, association analysis of common CH25H variants. PMID: 26857497
Infection with HCV causes up-regulation of interferon-inducible CH25H in vivo. PMID: 25999047
These results demonstrate that CH25H constitutes a primary innate response against hepatitis C virus infection through regulating host lipid metabolism. PMID: 25903345
Cholesterol 25-hydroxylase production by dendritic cells and macrophages is regulated by type I interferons. PMID: 20699362
Based on the results of genome-wide screens, along with biological studies, we selected three genes as candidates for AD risk factors: ATP-binding cassette transporter A1 (ABCA1), cholesterol 25-hydroxylase (CH25H) and cholesterol 24-hydroxylase (CH24H). PMID: 16157450

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Sterol desaturase family

Database Links

HGNC: 1907

OMIM: 604551

KEGG: hsa:9023

STRING: 9606.ENSP00000360918

UniGene: Hs.47357

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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