CPNE7 Antibody

Datasheet
Code CSB-PA866203LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9UBL6
Image
  • Western Blot
    Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Rat heart tissue
    All lanes: CPNE7 antibody at 3.6µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 71, 62 kDa
    Observed band size: 62 kDa

  • IHC image of CSB-PA866203LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of A549 cells with CSB-PA866203LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Copine-7 protein (254-393AA)
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Relevance Calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Copine-7 (Copine VII), CPNE7
Immunogen Species Human
Research Area Neuroscience
Target Names CPNE7
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes.
Subcellular Location Cytoplasm, Nucleus, Cell membrane
Protein Families Copine family
Tissue Specificity Expressed in the brain, testis, thymus and small intestine (PubMed:10534407, PubMed:12949241).
Database Links

HGNC: 2320

OMIM: 605689

KEGG: hsa:27132

STRING: 9606.ENSP00000268720

UniGene: Hs.461775

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