Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Rat heart tissue
All lanes: CPNE7 antibody at 3.6µg/ml
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 71, 62 kDa
Observed band size: 62 kDa
IHC image of CSB-PA866203LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CSB-PA866203LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).