D Antibody

Datasheet

Code	CSB-PA361132XA01FSF
Size	US$166
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Image	Western Blot Positive WB detected in: recombinant protein All lanes: D Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa
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Target Background

Product Details

Uniprot No.

P03712

Target Names

Alternative Names

Capsid decoration protein (Auxiliary protein D) (Gene product D) (gpD) (Major capsid protein D), D

Species Reactivity

Escherichia phage lambda

Immunogen

Recombinant Escherichia phage lambda D protein (1-110aa)

Immunogen Species

Escherichia phage lambda (Bacteriophage lambda)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Generation of the D polyclonal antibody involves rabbit immunization with recombinant Escherichia phage lambda gpD protein (1-110aa), triggering an immune response in the rabbit that prompts the production of specific antibodies within the rabbit's serum. Following affinity chromatography purification, the resultant D antibody exhibits reactivity with Escherichia phage lambda gpD protein and is extensively validated for use in ELISA and WB applications.

The Escherichia phage lambda gpD protein mainly acts as a decoration protein for the phage capsid. During the final stages of the phage lambda's life cycle, when DNA packaging is nearly complete, the gpD protein binds to the phage capsid, stabilizing the capsid structure and ensuring the proper formation of the phage particle. While it is not directly involved in DNA packaging, gpD is essential for packaging the full length of the phage's genetic material.

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Target Background

Function

Stabilizes the expansion of the capsid head shell after genome packaging. The packaging of viral genome in the procapsid triggers a dramatic reconfiguration of the capsid shell, expanding from roughly 50nm to 60nm while the capsid thickness decreases. 415 capsid decoration protein molecules cooperatively bind the expanded capsid, thereby stabilizing the mature capsid shell.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Lambda phage gpD family

Database Links

KEGG: vg:2703529

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