Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DGKA Polyclonal antibody

Uniprot No.

P23743

Target Names

DGKA

Alternative Names

80 kDa diacylglycerol kinase antibody; DAG kinase alpha antibody; DAGK antibody; DAGK1 antibody; DGK alpha antibody; DGK-alpha antibody; dgkA antibody; DGKA_HUMAN antibody; Diacylglycerol kinase alpha antibody; Diacylglycerol kinase; alpha 80kDa antibody; Diglyceride kinase alpha antibody; MGC12821 antibody; MGC42356 antibody; OTTHUMP00000242836 antibody; OTTHUMP00000242955 antibody; OTTHUMP00000244046 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Diacylglycerol kinase alpha protein (1-200AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function	Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.
Gene References into Functions	Diacylglycerol kinases alpha and zeta are up-regulated in cancer in cancer, and contribute towards tumor immune evasion and T cells clonal anergy. (Review) PMID: 27697466 DGKalpha isoform is highly expressed in the nuclei of human erythroleukemia cell line K562, and its nuclear activity drives K562 cells through the G1/S transition during cell cycle progression. PMID: 27731506 LIPFDGKA might serve as a potential possible biomarkers for diagnosis of gastric cancer, and their downregulation may bring new perspective into the investigation of gastric cancer prognosis PMID: 27498782 An abandoned compound that also inhibits serotonin receptors may have more translational potential as a DGKa inhibitor, but more potent and specific DGKa inhibitors are sorely needed PMID: 26420856 Decreased DNA methylation at this enhancer enables recruitment of the profibrotic transcription factor early growth response 1 (EGR1) and facilitates radiation-induced DGKA transcription in cells from patients later developing fibrosis. PMID: 26964756 Redundant and specialized roles for diacylglycerol kinases alpha and zeta in the control of T cell functions. PMID: 25921290 These data indicates the existence of a SDF-1alpha induced DGKalpha - atypical PKC - beta1 integrin signaling pathway, which is essential for matrix invasion of carcinoma cells. PMID: 24887021 DGKalpha generates phosphatidic acid to drive its own recruitment to tubular recycling endosomes via its interaction with MICAL-L1 PMID: 25248744 High diacylglycerol kinase alpha expression is associated with glioblastoma. PMID: 24158111 DGKalpha is involved in hepatocellular carcinoma progression by activation of the MAPK pathway. PMID: 22425622 DGK-alpha was more highly expressed in CD8-tumor-infiltrating T cellscompared with that in CD8non-tumor kidney-infiltrating lymphocytes. PMID: 22573804 Antigen-specific CD8-positive T cells from DGKalpha-deficient transgenic mice show enhanced expansion and increased cytokine production after lymphocytic choriomeningitis virus infection, yet DGK-deficient memory CD8+ T cells exhibit impaired expansion. PMID: 22271650 SAP-mediated inhibition of DGKalpha sustains diacylglycerol signaling, thereby regulating T cell activation PMID: 22048771 Diacylglycerol kinase alpha is a key regulator of the polarised secretion of exosomes. PMID: 21252909 findings further suggest that DGL-alpha and -beta may regulate neurite outgrowth by engaging temporally and spatially distinct molecular pathways PMID: 21493725 Defects in both polymorphonuclear neutrophil (PMN) transendothelial migration and PMN diacylglycerol kinase alpha signaling are implicated as disordered functions in subjects with localized aggressive periodontitis. PMID: 14734770 PPARgamma agonists upregulate DGKalpha production.This suppresses the diacylglycerol/protein-kinase-C signaling pathway. PMID: 15117825 DGKalpha is crucial for the control of cell activation and also for the regulation of the secretion of lethal exosomes, which in turn controls cell death. PMID: 15870081 ALK-mediated alphaDGK activation is dependent on p60src tyrosine kinase, with which alphaDGK forms a complex; alphaDGK activation is involved in the control of ALK-mediated mitogenic properties. PMID: 15928040 These results strongly suggest that DGKalpha is a novel positive regulator of NF-kappaB, which suppresses TNF-alpha-induced melanoma cell apoptosis. PMID: 17276726 diacylglycerol kinase alpha-conserved domains have a role in membrane targeting in intact T cells PMID: 17911109 2,3-dioleoylglycerol binds to a site on the alpha and zeta isoforms of diacylglycerol kinase that is exposed as a consequence of the substrate binding to the active site. PMID: 18004883 Lck-dependent tyrosine phosphorylation of diacylglycerol kinase alpha regulates its membrane association in T cells.( PMID: 18424699 These results strongly suggest that DGKalpha positively regulates TNF-alpha-dependent NF-kappaB activation via the PKCzeta-mediated Ser311 phosphorylation of p65/RelA. PMID: 19751727 Show More Hide All
Subcellular Location	Cytoplasm, cytosol.
Protein Families	Eukaryotic diacylglycerol kinase family
Tissue Specificity	Expressed in lymphocytes.
Database Links	HGNC: 2849 OMIM: 125855 KEGG: hsa:1606 STRING: 9606.ENSP00000328405 UniGene: Hs.524488

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Check Code：

Code	CSB-PA006832ESR1HU
Size	US$299Purchase it in Cusabio online store (only available for customers from the US)
Image	Western blot All lanes: DGKA antibody at 6.48µg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83, 14, 29 kDa Observed band size: 83 kDa Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA006832ESR1HU at dilution of 1:100
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DGKA Antibody

Product Details

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Target Background

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