DOK2 Antibody

Code CSB-PA002181
Size US$100
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  • Western Blot analysis of COLO205 cells using Dok-2 Polyclonal Antibody
  • Western Blot analysis of K562 LOVO cells using Dok-2 Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
DOK2
Alternative Names
Docking protein 2 56kDa antibody; Docking protein 2 antibody; DOK 2 antibody; DOK R antibody; DOK2 antibody; DOK2_HUMAN antibody; Downstream of tyrosine kinase 2 antibody; OTTHUMP00000161710 antibody; OTTHUMP00000224923 antibody; p56(dok 2) antibody; p56(dok-2) antibody; p56dok 2 antibody; p56DOK antibody; p56dok2 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthesized peptide derived from Human Dok-2 around the non-phosphorylation site of Y299.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
Gene References into Functions
  1. In this report, the authors demonstrated that the cellular adaptor proteins Dok-2 and Dok-1 are tyrosine phosphorylated upon herpes simplex virus 1 infection. In addition, herpes simplex virus 1 induced the selective degradation of Dok-2. Finally, Dok-2 interacts with herpes simplex virus 1 VP11/12, and that herpes simplex virus 1-induced tyrosine phosphorylation and degradation of Dok-2 require VP11/12. PMID: 28841444
  2. DOK2 and DOK3 expression was significantly reduced in HTLV-1-infected T cells. PMID: 27265473
  3. point mutations in DOK1 and DOK2 genes are detected with low frequency in chronic myelomonocytic leukemia but may have consequences for the function of the DOK2 PTB domain PMID: 25252871
  4. Taken together, these results reveal that Dok1 and Dok2 proteins are involved in an intrinsic negative feedback loop downstream of natural killer-cell-activating receptors in mouse and human. PMID: 24963146
  5. DOK2 as a tumor suppressor in EGFR-mutant lung adenocarcinoma PMID: 24255704
  6. DOK2 is a marker of poor prognosis in patients with gastric cancer after curative resection PMID: 22130622
  7. Data indicate there was no evidence of DOK2 somatic mutation in the leukemias analyzed. PMID: 21329978
  8. data indicate that DOK2 is altered in gastric (GC) and colorectal cancers (CRC) by loss of expressions; data indicate that somatic mutation of DOK2 may be rare in GC, CRC, breast cancer, prostate cancer and liver cancer PMID: 21749457
  9. Dok1 negatively regulates Dok2-mediated CD200R signaling through the recruitment of CrkL. PMID: 21078907
  10. Identification of DOK genes as lung tumor suppressors. PMID: 20139980
  11. identified tyrosine residue 1106 on Tie2 as an Angiopoietin 1-dependent autophosphorylation site that mediates binding and phosphorylation of the downstream-of-kinase-related (Dok-R) docking protein PMID: 12665569
  12. Dok-R and c-Abl interact in both a constitutive and inducible fashion and Dok-R influences the intracellular kinase and biological activity of c-Abl PMID: 12777393
  13. Dok-2 tyrosine phosphorylation was also found to be involved in collagen receptor, glycoprotein VI (GPVI), signaling as well as in outside-in signaling through the major platelet integrin, alpha IIIb beta 3 PMID: 14645010
  14. DOK1 and DOK2 interact with the Tec protein tyrosine kinase. PMID: 14647425
  15. Dok-R acts as an EGFR-recruited scaffolding molecule that processively assembles c-Src and Csk to attenuate signaling from the EGFR. PMID: 15831486
  16. Phosphotyrosine-binding mediated oligomerization of Dok-1 and Dok-2 represents an essential step for Dok phosphorylation and function. PMID: 16177091
  17. The data provide evidence that DOK2 protein has a role in regulating cell proliferation and differentiation and is positive regulators of the MAPK signaling pathway in this context. PMID: 16823827
  18. Dok-2 is a critical element of a linker for activation of T cells (LAT)-dependent negative feedback loop that attenuates early T-cell receptor (TCR) signal. PMID: 17043143
  19. results demonstrate differential modes of regulation of Dok1 and Dok2 in platelets, and raise the possibility that Dok2 plays an important role in integrin outside-in signaling through a physical and functional interaction with integrin alphaIIbbeta3 PMID: 17092301
  20. Dok-1/Dok-2 pleckstrin homology domains bind in vitro to the rare phosphoinositide species, phosphatidylinositol 5-phosphate PMID: 19299694
  21. CD200R inhibits the activation of human myeloid cells through direct recruitment of Dok2 and subsequent activation of RAS p21 protein activator 1. PMID: 19786546

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Protein Families
DOK family, Type A subfamily
Tissue Specificity
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Database Links

HGNC: 2991

OMIM: 604997

KEGG: hsa:9046

STRING: 9606.ENSP00000276420

UniGene: Hs.71215

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