DPH5 Antibody

Datasheet

Code	CSB-PA862001LA01HU
Size	US$166
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Image	IHC image of CSB-PA862001LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of SH-SY5Y cells with CSB-PA862001LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DPH5 Polyclonal antibody

Uniprot No.

Q9H2P9

Target Names

DPH5

Alternative Names

DPH5 antibody; AD-018 antibody; CGI-30 antibody; HSPC143 antibody; NPD015Diphthine methyl ester synthase antibody; EC 2.1.1.314 antibody; Diphthamide biosynthesis methyltransferase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Diphthine methyl ester synthase protein (138-235AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DPH5 Antibody (Product code: CSB-PA862001LA01HU) is Non-conjugated. For DPH5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA862001LB01HU	DPH5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA862001LC01HU	DPH5 Antibody, FITC conjugated
Biotin	CSB-PA862001LD01HU	DPH5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

Gene References into Functions

The yeast and Chinese hamster homologs of human DPH5 catalyze the trimethylation step in the biosynthesis of diphthamide PMID: 15485916

Protein Families

Diphthine synthase family

Database Links

HGNC: 24270

OMIM: 611075

KEGG: hsa:51611

STRING: 9606.ENSP00000359127

UniGene: Hs.440776

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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