ELP3 Antibody

Datasheet

Code	CSB-PA884499LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate All lanes: ELP3 antibody at 3.82µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63, 61, 50, 53 kDa Observed band size: 63 kDa IHC image of CSB-PA884499LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA884499LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating ELP3 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA884499LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA884499LA01HU (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ELP3 Polyclonal antibody

Uniprot No.

Q9H9T3

Target Names

ELP3

Alternative Names

DKFZp761L098 antibody; Elongation protein 3 homolog (S. cerevisiae) antibody; Elongation protein 3 homolog antibody; ELONGATION PROTEIN 3; S. CEREVISIAE; HOMOLOG OF antibody; elongator acetyltransferase complex subunit 3 antibody; Elongator complex protein 3 antibody; elp3 antibody; ELP3_HUMAN antibody; FLJ10422 antibody; hELP3 antibody; Hypothetical protein DKFZp761L098 antibody; Kat9 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Elongator complex protein 3 protein (1-85AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ELP3 Antibody (Product code: CSB-PA884499LA01HU) is Non-conjugated. For ELP3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA884499LB01HU	ELP3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA884499LC01HU	ELP3 Antibody, FITC conjugated
Biotin	CSB-PA884499LD01HU	ELP3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex.

Gene References into Functions

and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
Promoter hypermethylation is an important mechanism of the transcriptional inactivation of ELP3 in invasive ductal breast carcinoma. PMID: 25148870
Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress. PMID: 24769394
data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function PMID: 22216241
The results of this study uncover a novel role for Elp3 in the regulation of synaptic bouton expansion during neurogenesis that may be linked with a requirement for sleep. PMID: 20626565
Elp3 promotes the acetylation of alpha-tubulin in microtubules in neurological disorders. PMID: 20036197
We used hELP3 antisense oligonucl. to knock down hELP3 gene. The results showed that reduction of hELP3 mRNA and protein caused a suppression of HSP70-2 and histone H3 hypoacetylation. PMID: 17558451
Allelic variants of ELP3 were associated with amyotrophic lateral sclerosis in three populations comprising 1483 people. PMID: 18996918
ELP3 localises to mitochondria in HeLa cells, actin-like filaments, and actin-rich sites at the edges of spreading cells. This suggests that ELP3 and the ELONGATOR complex may play a role in mitochondrial function, actin organisation, and cell motility. PMID: 19429107

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Involvement in disease

ELP3 genetic variations may be associated with an increased risk for neurodegeneration and motor neuron diseases.

Subcellular Location

Cytoplasm. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm. Nucleus.

Protein Families

ELP3 family

Tissue Specificity

Expressed in the cerebellum and spinal motor neurons.

Database Links

HGNC: 20696

OMIM: 612722

KEGG: hsa:55140

STRING: 9606.ENSP00000256398

UniGene: Hs.491336

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Protein Acetylation: An Important Post-translational Modification in Eukaryotic Cells A Comprehensive Exploration into Amyotrophic Lateral Sclerosis Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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