FAM83D Antibody

Datasheet
Code CSB-PA887979LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9H4H8
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate
    All lanes: FAM83D antibody at 4.4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 65, 63 kDa
    Observed band size: 65 kDa

  • Immunofluorescence staining of HepG2 cells with CSB-PA887979LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Protein FAM83D protein (339-470AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
Relevance Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed:24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed:25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Protein FAM83D (Spindle protein CHICA), FAM83D, C20orf129
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names FAM83D
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC
Subcellular Location Cytoplasm, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, spindle pole
Protein Families FAM83 family
Database Links

HGNC: 16122

KEGG: hsa:81610

STRING: 9606.ENSP00000217429

UniGene: Hs.726442

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