FARP1 Antibody

Code CSB-PA897492LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: A375 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
    All lanes: FARP1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 119, 123, 15 kDa
    Observed band size: 119 kDa

  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA897492LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA897492LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA897492LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunofluorescent analysis of A549 cells using CSB-PA897492LA01HU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG

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Product Details

Description

The generation of the product CSB-PA897492LA01HU is through immunizing rabbits with the peptide corresponding to amino acids 1-300 of the recombinant human FARP1 protein. The production is a polyclonal antibody and occurs as an unconjugated IgG. It is reactive with human FARP1 protein, which participates in regulating neuronal development through interacting with cell-surface proteins such as class A Plexins and SynCAM 1. Its purity is greater than 95% using protein G purified. And this FARP1 antibody has been validated for use in ELISA, WB, IHC, and IF applications.

Full Product Name Rabbit anti-Homo sapiens (Human) FARP1 Polyclonal antibody
Uniprot No. Q9Y4F1
Target Names FARP1
Alternative Names CDEP antibody; Chondrocyte derived ezrin like protein antibody; Chondrocyte-derived ezrin-like protein antibody; FARP1 antibody; FARP1_HUMAN antibody; FERM RhoGEF and pleckstrin domain containing protein 1 antibody; FERM antibody; FERM RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) antibody; FERM; ARHGEF; and pleckstrin domain protein 1 antibody; PH domain-containing family C member 2 antibody; Pleckstrin homology domain-containing family C member 2 antibody; PLEKHC2 antibody; PPP1R75 antibody; protein phosphatase 1; regulatory subunit 75 antibody; RhoGEF and pleckstrin domain-containing protein 1 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human FERM, ARHGEF and pleckstrin domain-containing protein 1 protein (1-300AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Functions as guanine nucleotide exchange factor for RAC1. May play a role in semaphorin signaling. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses (By similarity).
Gene References into Functions
  1. We have reported here for the first time a reduced activity of both Rac1 and Cdc42 in human pheochromocytoma resection as well as tumor-associated expression changes of FARP1, ARHGEF1, and ARHGAP36 PMID: 26911374
Subcellular Location Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell junction, synapse, Cell junction, synapse, synaptosome, Cytoplasm, cytosol, Cell projection, filopodium, Cell projection, dendrite, Cell projection, dendritic spine
Tissue Specificity Detected in cAMP-treated chondrocytes, but not in untreated chondrocytes. Detected in fetal brain, heart and spleen, and in adult testis, kidney and lung.
Database Links

HGNC: 3591

OMIM: 602654

KEGG: hsa:10160

STRING: 9606.ENSP00000322926

UniGene: Hs.403917

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