FAU Antibody

Datasheet
Code CSB-PA008447LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. P62861
Image
  • IHC image of CSB-PA008447LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of A549 cells with CSB-PA008447LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human 40S ribosomal protein S30 protein (1-59AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200
Relevance cytosol, cytosolic small ribosomal subunit, extracellular space, nucleoplasm, small ribosomal subunit, RNA binding, structural constituent of ribosome, antibacterial humoral response, antimicrobial humoral immune response mediated by antimicrobial peptide, defense response to Gram-positive bacterium
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias 40S ribosomal protein S30, Small ribosomal subunit protein eS30, FAU
Immunogen Species Human
Research Area Others
Target Names FAU
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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