FBL Antibody

Code CSB-PA919400
Size US$297
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  • Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) FBL Polyclonal antibody
Uniprot No.
Target Names
FBL
Alternative Names
34 kD nucleolar scleroderma antigen antibody; 34 kDa nucleolar scleroderma antigen antibody; Fbl antibody; FBRL_HUMAN antibody; FIB antibody; FIB1 antibody; FLRN antibody; Histone-glutamine methyltransferase antibody; Nop1p antibody; RNA U3 small nucleolar interacting protein 1 antibody; RNU3IP1 antibody; rRNA 2' O methyltransferase fibrillarin antibody; rRNA 2'-O-methyltransferase fibrillarin antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Peptide sequence around aa.297~301( L-T-L-E-P) derived from Human Fibrillarin
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Purification Method
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.
Gene References into Functions
  1. Fibrillarin acts as a central node in a regulatory network engaged in imparting immunity against bacterial pathogens. PMID: 30190478
  2. Nucleolar Methyltransferase Fibrillarin: Evolution of Structure and Functions. PMID: 27682166
  3. FBL overexpression contributes to tumorigenesis and is associated with poor survival in patients with breast cancer. PMID: 24029231
  4. NS1 protein of the human H3N2 virus interacts primarily via the C-terminal NLS2/NoLS and to a minor extent via the N-terminal NLS1 with the main nucleolar proteins, nucleolin, B23 and fibrillarin. PMID: 22909121
  5. p32 is a new rRNA maturation factor involved in the remodeling from pre-90S particles to pre-40S and pre-60S particles that requires the exchange of FBL for Nop52. PMID: 21536856
  6. Increased levels of exogenous BRAG2 in nucleoli result in redistribution of FBL to the nucleolar periphery. PMID: 17461797
  7. Our data suggest that fibrillarin would play a critical role in the maintenance of nuclear shape and cellular growth. PMID: 17603021
  8. Evidence that BIG1 and nucleolin, but not fibrillarin, can be present with p62 at the nuclear envelope confirms the presence of BIG1 and nucleolin in dynamic molecular complexes that change in composition while moving through nuclei PMID: 18292223
  9. Data demonstrate that fibrillarin and Nop56 directly interact in vivo, and that this interaction is indispensable for the association of both proteins with the box C/D snoRNPs. PMID: 19331828

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Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Fibrillar region of the nucleolus.
Protein Families
Methyltransferase superfamily, Fibrillarin family
Database Links

HGNC: 3599

OMIM: 134795

KEGG: hsa:2091

STRING: 9606.ENSP00000221801

UniGene: Hs.299002

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