FRYL Antibody

Datasheet

Code	CSB-PA009007LA01HU
Size	US$166
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Image	IHC image of CSB-PA009007LA01HU diluted at 1:600 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA009007LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FRYL Polyclonal antibody

Uniprot No.

O94915

Target Names

FRYL

Alternative Names

2010313D22Rik antibody; 2310004H21Rik antibody; 2510002A14Rik antibody; 9030227G01Rik antibody; AF4P12 antibody; AI930088 antibody; ALL fused gene from chromosome 4p12 antibody; ALL1-fused gene from chromosome 4p12 protein antibody; DKFZp686E205 antibody; FLJ161772 antibody; FRY like antibody; FRYL antibody; FRYL_HUMAN antibody; Furry homolog like (Drosophila) antibody; Furry homolog like antibody; Furry like antibody; KIAA0826 antibody; mKIAA0826 antibody; Protein furry homolog-like antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein furry homolog-like protein (42-739AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FRYL Antibody (Product code: CSB-PA009007LA01HU) is Non-conjugated. For FRYL Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA009007LB01HU	FRYL Antibody, HRP conjugated	ELISA
FITC	CSB-PA009007LC01HU	FRYL Antibody, FITC conjugated
Biotin	CSB-PA009007LD01HU	FRYL Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching. May function as a transcriptional activator.

Gene References into Functions

Thus, t(4;11)(p12;q23) with MLL and FRYL involvement represents a new recurring 11q23 translocation, to date seen only in therapy-related acute leukemias. PMID: 17854671

Involvement in disease

A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.

Protein Families

Furry protein family

Tissue Specificity

Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.

Database Links

HGNC: 29127

KEGG: hsa:285527

STRING: 9606.ENSP00000351113

UniGene: Hs.595553

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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