Fn1 Antibody

^{Rare Species}

Datasheet

Code	CSB-PA008759HA01RA
Size	US$166
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Image	IHC image of CSB-PA008759HA01RA diluted at 1:200 and staining in paraffin-embedded rat kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Full Product Name

Rabbit anti-Rattus norvegicus (Rat) Fn1 Polyclonal antibody

Uniprot No.

P04937

Target Names

Fn1

Alternative Names

Fibronectin (FN) [Cleaved into: Anastellin], Fn1

Raised in

Rabbit

Species Reactivity

Rat

Immunogen

Recombinant Rat Fibronectin protein (1175-1451aa)

Immunogen Species

Rattus norvegicus (Rat)

Conjugate

Non-conjugated

The Fn1 Antibody (Product code: CSB-PA008759HA01RA) is Non-conjugated. For Fn1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA008759HB01RA	Fn1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA008759HC01RA	Fn1 Antibody, FITC conjugated
Biotin	CSB-PA008759HD01RA	Fn1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:100-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.

Database Links

STRING: 10116.ENSRNOP00000019772

UniGene: Rn.1604

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Related pathways

AGE-RAGE signaling pathway in diabetic complications PI3K-Akt signaling pathway Cell markers Regulation of actin cytoskeleton Focal adhesion

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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