GATB Antibody

Datasheet

Code	CSB-PA017792LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate All lanes: GATB antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa IHC image of CSB-PA017792LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA017792LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GATB Polyclonal antibody

Uniprot No.

O75879

Target Names

GATB

Alternative Names

GATB antibody; PET112 antibody; PET112L antibody; HSPC199Glutamyl-tRNA(Gln) amidotransferase subunit B antibody; mitochondrial antibody; Glu-AdT subunit B antibody; EC 6.3.5.- antibody; Cytochrome c oxidase assembly factor PET112 homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial protein (301-383AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GATB Antibody (Product code: CSB-PA017792LA01HU) is Non-conjugated. For GATB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017792LB01HU	GATB Antibody, HRP conjugated	ELISA
FITC	CSB-PA017792LC01HU	GATB Antibody, FITC conjugated
Biotin	CSB-PA017792LD01HU	GATB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).

Gene References into Functions

Studies showed in vitro Gln-tRNA(Gln) formation catalyzed by the recombinant mtGluRS and hGatCAB. PMID: 19805282

Subcellular Location

Mitochondrion.

Protein Families

GatB/GatE family, GatB subfamily

Tissue Specificity

Predominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart.

Database Links

HGNC: 8849

OMIM: 603645

KEGG: hsa:5188

STRING: 9606.ENSP00000263985

UniGene: Hs.119316

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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