GNG12 Antibody

Datasheet

Code	CSB-PA883362OA01HU
Size	US$166
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Image	IHC image of CSB-PA883362OA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of PC-3 cells with CSB-PA883362OA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GNG12 Polyclonal antibody

Uniprot No.

Q9UBI6

Target Names

GNG12

Alternative Names

GNG12Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence from Human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 protein (2-17AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GNG12 Antibody (Product code: CSB-PA883362OA01HU) is Non-conjugated. For GNG12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA883362OB01HU	GNG12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA883362OC01HU	GNG12 Antibody, FITC conjugated
Biotin	CSB-PA883362OD01HU	GNG12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

G protein gamma family

Database Links

HGNC: 19663

OMIM: 615405

KEGG: hsa:55970

STRING: 9606.ENSP00000360021

UniGene: Hs.431101

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Chemokine signaling pathway MAPK signaling pathway PI3K-Akt signaling pathway Regulation of actin cytoskeleton Ras signaling pathway Apelin signaling pathway Glutamatergic synapse

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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