GNL3L Antibody

Code CSB-PA002732
Size US$100
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  • Western Blot analysis of HT29 cells using GNL3L Polyclonal Antibody
  • Western Blot analysis of NIH-3T3 LOVO cells using GNL3L Polyclonal Antibody
  • Western blot analysis of mouse-brain HELA KB SH-SY5Y 293T 3T3 Mouse-kidney lysis using GNL3L antibody.
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Product Details

Uniprot No.
Target Names
GNL3L
Alternative Names
GNL3LGuanine nucleotide-binding protein-like 3-like protein antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Synthesized peptide derived from the Internal region of Human GNL3L.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP.
Gene References into Functions
  1. GNL3L-LDOC1 interplay regulates cell proliferation through the modulation of NF-kappaB pathway during tumorigenesis. PMID: 27764577
  2. the present study provides evidence that GNL3L is exported from the nucleus in CRM1 dependent manner and the nuclear localization of GNL3L is important to promote 'S' phase progression during cell proliferation. PMID: 26274615
  3. GNL3L depletion impairs ribosome production without inducing appreciable DNA damage. During evolution, GNL3L retained the role of the ancestral gene in ribosome biosynthesis, whereas the paralogous nucleostemin acquired a novel genome-protective function. PMID: 24610951
  4. differences in the relative nucleostemin protein and mRNA levels may reflect the degree of proliferation of mesnchymal stem cells and can be used to characterize in vitro expansion capabilities PMID: 21063916
  5. GNL3L bound MDM2 in vivo, stabilizing MDM2 and preventing its ubiquitylation. GNL3L depletion upregulated specific p53 targets (Bax, 14-3-3sigma and p21). PMID: 21132010
  6. analysis uncovers an important role for Grn1p/GNL3L within this unique group of nucleolar GTPases PMID: 16251348
  7. GNL3L is composed of distinct modules, each of which plays a specific role in molecular interactions for its nucleolar retention and subsequent function(s) within the nucleolus PMID: 17034816

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Subcellular Location
Nucleus, nucleolus.
Protein Families
TRAFAC class YlqF/YawG GTPase family
Database Links

HGNC: 25553

OMIM: 300873

KEGG: hsa:54552

STRING: 9606.ENSP00000338573

UniGene: Hs.654677

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