GOLGB1 Antibody

Datasheet

Code	CSB-PA622788LA01HU
Size	US$166
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Image	IHC image of CSB-PA622788LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA622788LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GOLGB1 Polyclonal antibody

Uniprot No.

Q14789

Target Names

GOLGB1

Alternative Names

372 kDa Golgi complex associated protein antibody; 372 kDa Golgi complex-associated protein antibody; GCP antibody; GCP372 antibody; Giantin antibody; GOGB1_HUMAN antibody; GOLGB1 antibody; Golgi autoantigen golgin subfamily a member 1 antibody; Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1 antibody; Golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody; Golgi complex associated protein 372kDa antibody; Golgi complex-associated protein 372kDa antibody; Golgin B1 antibody; Golgin B1, golgi integral membrane protein antibody; Golgin subfamily B member 1 antibody; GOLIM1 antibody; Macrogolgin antibody; MGC33154 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Golgin subfamily B member 1 protein (1764-1869AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GOLGB1 Antibody (Product code: CSB-PA622788LA01HU) is Non-conjugated. For GOLGB1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA622788LB01HU	GOLGB1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA622788LC01HU	GOLGB1 Antibody, FITC conjugated
Biotin	CSB-PA622788LD01HU	GOLGB1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May participate in forming intercisternal cross-bridges of the Golgi complex.

Gene References into Functions

Single nucleotide polymorphisms (SNPs) rs1035798 in RAGE gene, rs2073617 and rs2073618 in TNFRSF11B, and rs3732410 in Golgb1 will be investigated on whether there is an association with hemorrhagic stroke (HS) in Chinese population. PMID: 26664786
Partial depletion of giantin or of WDR34 leads to an increase in cilia length consistent with the concept that giantin acts through dynein-2. PMID: 24046448
the spatial organization of the Golgi ribbon is mediated by giantin, which also plays a role in cargo transport and sugar modifications PMID: 23555793
unbiased whole-genome search for genetic modifiers of stroke risk in sickle cell anemia was performed; mutation in GOLGB1 (Y1212C) and mutation in ENPP1 (K173Q) were confirmed as having significant associations with a decreased risk for stroke PMID: 23422753
Data report the characterization of a mammalian coiled-coil protein, CASP, a Golgi protein that shares with giantin a conserved histidine in its transmembrane domain. PMID: 12429822
Giantin binds to Rab6A and Rab1 proteins. PMID: 17475246

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Subcellular Location

Golgi apparatus membrane; Single-pass type I membrane protein.

Database Links

HGNC: 4429

OMIM: 602500

KEGG: hsa:2804

STRING: 9606.ENSP00000341848

UniGene: Hs.213389

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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