GPR27 Antibody

Datasheet
Code CSB-PA885717LA01HU
See More Details
Size US$299
Uniprot No. Q9NS67
Image
  • IHC image of CSB-PA885717LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA885717LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Probable G-protein coupled receptor 27 protein (203-285AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200
Relevance Orphan receptor. Possible candidate for amine-like G-protein coupled receptor.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Probable G-protein coupled receptor 27 (Super conserved receptor expressed in brain 1), GPR27, SREB1
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names GPR27
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Orphan receptor. Possible candidate for amine-like G-protein coupled receptor.
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Highly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb t
Database Links

HGNC: 4482

OMIM: 605187

KEGG: hsa:2850

STRING: 9606.ENSP00000303149

UniGene: Hs.591653

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