Product Details

Uniprot No.

Target Names

GPSM1

Alternative Names

Activator of G-protein signaling 3 antibody; AGS3 antibody; AGS3 homolog antibody; C10a antibody; G protein signaling modulator 1 (AGS3 like; C. elegans) antibody; G-protein-signaling modulator 1 antibody; GPSM1 antibody; GPSM1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Human GPSM1

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB,IHC

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.

Gene References into Functions

The G-protein Regulatory motif of AGS3 is critical for regulating MUC1/Muc1 expression and cytokine production in the inflammatory microenvironment. PMID: 27270970
Data support the notion that the Galpha, but not Gbetagamma, arm of the Gi/o signalling is involved in TRPC4 activation and unveil new roles for RGS and AGS3 in fine-tuning TRPC4 activities. PMID: 26987813
Activator of G-protein Signaling 3 is an important regulator of esophageal squamous cell carcinoma proliferation PMID: 25812748
Results confirmed that Ric-8A can directly bind to AGS3S but failed to facilitate Galpha(i)-induced suppression of adenylyl cyclase, suggesting that it may not serve as a guanine exchange factor for AGS3/Galpha(i/o)-GDP complex in a cellular environment. PMID: 25480567
High GPSM1 gene methylation is associated with gastric tumor aggressiveness. PMID: 25740824
Data identified three new loci for type 2 diabetes with genome-wide significance: MIR129-LEP, GPSM1 and SLC16A13. PMID: 23945395
Our data indicate that decreased expression of AGS3 is correlated with reduced levels of p-CREB in the apoptotic model. The negative role of AGS3 in cell apoptosis was further confirmed by knocking down AGS3. PMID: 24307516
these results indicate that GRK6 complexes with AGS3-Galphai2 to regulate CXCR2-mediated leukocyte functions at different levels, including downstream effector activation, receptor trafficking, and expression at the cell membrane. PMID: 24510965
These results provide mechanistic insights into how reversible modulation of Galpha(i3) activity by AGS3 and GIV maintains the delicate equilibrium between promotion and inhibition of autophagy. PMID: 21209316
AGS3 receptor coupling to both Galphabetagamma and GPR-Galpha(i) offer additional flexibility for systems to respond and adapt to challenges and orchestrate complex behaviors PMID: 20716524
unlike wild-type AGS3, over-expression of an AGS3 mutant lacking this modification fails to enhance macroautophagic activity. These observations imply that AGS3 phosphorylation may participate in the modulation of macroautophagy PMID: 20126274
These data present AGS3, G-proteins, and mInsc as candidate proteins involved in regulating cellular stress associated with protein-processing pathologies. PMID: 20065032
AGS3 is involved in an early event during the autophagic pathway probably prior to the formation of the autophagosome. PMID: 12642577
cortical localization in mitotic cell culture systems and requirement for normal cell cycle progression PMID: 12925752
data support a model wherein AGS3 modulates the protein trafficking along the TGN/plasma membrane/endosome loop PMID: 17991770
The GPR motif in this protein and other proteins is actually associated with activity as a GDI - guanine nucleotide dissociation inhibitor and not GTPase activator as initially suggested. PMID: 10969064

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Subcellular Location

Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

GPSM family

Tissue Specificity

Expressed in intestinal cells.

Database Links

HGNC: 17858

OMIM: 609491

KEGG: hsa:26086

STRING: 9606.ENSP00000392828

UniGene: Hs.239370