HAO1 Antibody

Datasheet

Code	CSB-PA891938LA01HU
Size	US$166
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Image	IHC image of CSB-PA891938LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA891938LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HAO1 Polyclonal antibody

Uniprot No.

Q9UJM8

Target Names

HAO1

Alternative Names

(S) 2 hydroxy acid oxidase antibody; Glycolate oxidase antibody; GOX antibody; GOX1 antibody; HAO1 antibody; HAOX1 antibody; HAOX1_HUMAN antibody; Hydroxyacid oxidase 1 antibody; MGC142225 antibody; MGC142227 antibody; OTTHUMP00000030231 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Hydroxyacid oxidase 1 protein (161-273AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HAO1 Antibody (Product code: CSB-PA891938LA01HU) is Non-conjugated. For HAO1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA891938LB01HU	HAO1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891938LC01HU	HAO1 Antibody, FITC conjugated
Biotin	CSB-PA891938LD01HU	HAO1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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■ Q&A

Specifically regarding your HAO-1 Antibodies. I've noticed that most of them say the immunogen is a recombinant Human Hydroxyacid oxidase 1 protein (161-273AA) is there a reason why this section of the protein was used?

We use this section for the antigen preparation to produce the rabbit polyclonal antibodies because amino acids 161-273 of HAO1 offer the best immunogenicity based on our analysis of the protein.

Target Background

Function

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.

Gene References into Functions

The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067

Subcellular Location

Peroxisome.

Protein Families

FMN-dependent alpha-hydroxy acid dehydrogenase family

Tissue Specificity

Liver.

Database Links

HGNC: 4809

OMIM: 605023

KEGG: hsa:54363

STRING: 9606.ENSP00000368066

UniGene: Hs.193640

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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