HIST1H1D (Ab-140) Antibody

Datasheet
Code CSB-PA010379PA140nforHU
Size US$319
Uniprot No. P16402
Image
  • Western Blot
    Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, PC3 whole cell lysate
    All lanes: HIST1H1D antibody at 1:166
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 23 kDa
    Observed band size: 32 kDa

  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010379PA140nforHU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA010379PA140nforHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunogen Peptide sequence around site of Lys (140) derived from Human Histone H1.3.
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:100-1:1000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H1.3 (Histone H1c) (Histone H1s-2), HIST1H1D, H1F3
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H1D
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4717

OMIM: 142210

KEGG: hsa:3007

STRING: 9606.ENSP00000244534

UniGene: Hs.136857

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