HIST1H2BK Antibody

Datasheet

Code	CSB-PA010411LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA010411LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010411LA01HU at dilution of 1:100 Immunofluorescence staining of 293 cells with CSB-PA010411LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HIST1H2BK Polyclonal antibody

Uniprot No.

O60814

Target Names

HIST1H2BK

Alternative Names

H2BC12 antibody; H2BFT antibody; HIRIP1 antibody; HIST1H2BK antibody; Histone H2B type 1-K antibody; H2B K antibody; HIRA-interacting protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Histone H2B type 1-K protein (2-126AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HIST1H2BK Antibody (Product code: CSB-PA010411LA01HU) is Non-conjugated. For HIST1H2BK Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA010411LB01HU	HIST1H2BK Antibody, HRP conjugated	ELISA
FITC	CSB-PA010411LC01HU	HIST1H2BK Antibody, FITC conjugated
Biotin	CSB-PA010411LD01HU	HIST1H2BK Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Gene References into Functions

Histone H2B antimicrobial peptides exhibit antibacterial activity and contribute to the formation of the antimicrobial barrier of the colonic epithelium PMID: 15019208

Subcellular Location

Nucleus. Chromosome.

Protein Families

Histone H2B family

Database Links

HGNC: 13954

OMIM: 615045

KEGG: hsa:85236

STRING: 9606.ENSP00000349430

UniGene: Hs.437275

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What Are Histones: Unraveling Their Critical Roles in Disease Pathogenesis Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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