Histone H2B type 1-K Antibody

Code CSB-RA010411A0HU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
    All lanes: Histone H2B type 1-K antibody at 0.16μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 14 KDa
    Observed band size: 14 KDa
  • IHC image of CSB-RA010411A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details

Uniprot No. O60814
Target Names HIST1H2BK
Alternative Names H2BC12 antibody; H2BFT antibody; HIRIP1 antibody; HIST1H2BK antibody; Histone H2B type 1-K antibody; H2B K antibody; HIRA-interacting protein 1 antibody
Species Reactivity Human
Immunogen A synthesized peptide
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 4G10
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:5000-1:10000
IHC 1:50-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; FUNCTION
Gene References into Functions
  1. Histone H2B antimicrobial peptides exhibit antibacterial activity and contribute to the formation of the antimicrobial barrier of the colonic epithelium PMID: 15019208
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2B family
Database Links

HGNC: 13954

OMIM: 615045

KEGG: hsa:85236

STRING: 9606.ENSP00000349430

UniGene: Hs.437275

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