Histone H2B type 1-K Recombinant Monoclonal Antibody

Code CSB-RA010411A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
    All lanes: Histone H2B type 1-K antibody at 0.16μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 14 KDa
    Observed band size: 14 KDa
  • IHC image of CSB-RA010411A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details

Uniprot No.
Target Names
HIST1H2BK
Alternative Names
H2BC12 antibody; H2BFT antibody; HIRIP1 antibody; HIST1H2BK antibody; Histone H2B type 1-K antibody; H2B K antibody; HIRA-interacting protein 1 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
4G10
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:5000-1:10000
IHC 1:50-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CUSABIO got the DNA sequence of the histone H2B type 1-K monoclonal antibody that was produced from the splenocytes derived from animals with the human histone H2B type 1-K synthesized peptide immunization. The DNA sequence was cloned into the plasmid and then transfected into cell lines for in vitro expression. The product is the histone H2B type 1-K recombinant monoclonal antibody. It is a rabbit IgG antibody purified using the affinity-chromatography method. This histone H2B type 1-K antibody is recommended for ELISA, WB, and IHC applications and detects the histone H2B type 1-K protein from human sources.

A study by Shih-Yi Lin et al. has reported that Alpha 1-Antitrypsin and H2B1K are diagnostic and prognostic biomarkers for urothelial carcinoma after proteome profiling of urinary exosomes. H2B1K has been demonstrated to have prognostic significance, as 3-fold increases in its levels properly predicted recurrence.

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Target Background

Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Gene References into Functions
  1. Histone H2B antimicrobial peptides exhibit antibacterial activity and contribute to the formation of the antimicrobial barrier of the colonic epithelium PMID: 15019208
Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H2B family
Database Links

HGNC: 13954

OMIM: 615045

KEGG: hsa:85236

STRING: 9606.ENSP00000349430

UniGene: Hs.437275

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