HPN Antibody

Datasheet

Code	CSB-PA010704LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse liver tissue All lanes: HPN antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa IHC image of CSB-PA010704LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA010704LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HPN Polyclonal antibody

Uniprot No.

P05981

Target Names

HPN

Alternative Names

HPN; TMPRSS1; Serine protease hepsin; Transmembrane protease serine 1

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Serine protease hepsin protein (294-413AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HPN Antibody (Product code: CSB-PA010704LA01HU) is Non-conjugated. For HPN Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA010704LB01HU	HPN Antibody, HRP conjugated	ELISA
FITC	CSB-PA010704LC01HU	HPN Antibody, FITC conjugated
Biotin	CSB-PA010704LD01HU	HPN Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The HPN polyclonal antibody is a rabbit antibody that specifically recognizes HPN proteins from human, mouse, and rat samples. Its immunogen is the recombinant human serine protease hepsin protein (294-413aa). It has been validated for use in ELISA, WB, IHC, and IF assays. The antibody has been purified using protein G and has a purity level of over 95%.

Hepsin is a type II transmembrane serine protease that plays a key role in several physiological processes, including the regulation of blood coagulation, cell growth and differentiation, and tumor progression. It is involved in the progression of several types of cancer, including prostate, breast, and ovarian cancer.

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Target Background

Function

Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL. Plays a role in cell growth and maintenance of cell morphology. Plays a role in the proteolytic processing of ACE2. Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization.

Gene References into Functions

Study showed was that Hepsin was downregulated in 78% of gastric cancer. Its high expression seems to predict poor prognosis. These results predicted that hepsin protein expression was one of the significant and independent prognostic factors for overall survival of Gastric Cancer. PMID: 27841306
Genetic variation in/near the gene encoding for hepsin protein may influence risk of bipolar disorder and (at least for the rs62122114-A allele) may have functional impact (i.e. differential expression) as evidenced by serum HPN protein expression. PMID: 28886448
significant negative association was found between hepsin expression in endometrial carcinoma cases regarding the grade and the size of tumors as well as myometrial invasion PMID: 26990747
These data demonstrate that the membrane-bound serine protease hepsin is the enzyme responsible for the physiological cleavage of uromodulin. PMID: 26673890
The Hepsin pathway acts in concert with Wnt pathway to promote prostate cancer progression. PMID: 26139199
Low expression levels of hepsin and TMPRSS3 are associated with poor breast cancer survival PMID: 26014348
Hepsin suppressed CDK11p58 internal ribosome entry site activity in prostate cancer cells by modulating UNR expression and eIF-2alpha phosphorylation. PMID: 25576733
The results of this study suggest that, in Korean men, some polymorphisms in the HPN gene might be associated with the risk of developing prostate cancer. PMID: 22665141
hepsin expression is frequently up-regulated in breast cancer tissues, which is associated with tumor growth and progression PMID: 21383634
These findings suggest that the MSP/RON signaling pathway may be regulated by hepsin in tissue homeostasis and in disease pathologies, such as in cancer and immune disorders. PMID: 21875933
Elevated levels of hepsin interfere with cell adhesion and viability in the background of prostate cancer as well as other tissue types, the details of which depend on the microenvironment provided. PMID: 21750652
Hepsin activity was inhibited by anthralin and increased by resveratrol. PMID: 20673210
Germline genetic variation of HPN does not seem to contribute to risk of prostate cancer or prognosis. PMID: 20166135
Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor. PMID: 19911255
Hepsin and maspin are inversely expressed in laser capture microdissectioned prostate cancer PMID: 12629351
Hepsin is functionally linked to hepatocyte growth factor/MET pathway, which may contribute to prostate cancer progression. PMID: 15792801
A major 11-locus haplotype is significantly associated with prostate cancer, which provides further support that HPN is a potentially important candidate gene involved in prostate cancer susceptibility. PMID: 16783571
the ability of hepsin to efficiently activate pro-uPA suggests that it may initiate plasmin-mediated proteolytic pathways at the tumor/stroma interface that lead to basement membrane disruption and tumor progression PMID: 16908524
Our findings suggest that the balance between hepsin and its inhibitor, HAI-2, may have prognostic value in RCC. PMID: 17309599
We analyzed all the common variations using tag SNP in the HPN and LTBP4 genes for association with IA in 390 patients and 642 controls in the Dutch population. PMID: 18487557
Hepsin showed potential inhibitory effects mediated by the induction of 14-3-3sigma expression which leads to both cell cycle arrest at the G2/M phase PMID: 18698500
Ln-332 may be one mechanism by which hepsin promotes prostate tumor progression and metastasis, possibly by up-regulating prostate cancer cell motility. PMID: 18784072

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Subcellular Location

Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein.

Protein Families

Peptidase S1 family

Tissue Specificity

Detected in liver and kidney.

Database Links

HGNC: 5155

OMIM: 142440

KEGG: hsa:3249

STRING: 9606.ENSP00000262626

UniGene: Hs.182385

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