IFIT2 Antibody

Datasheet

Code	CSB-PA011021LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate, Rat liver tissue All lanes: IFIT2 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa IHC image of CSB-PA011021LA01HU diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA011021LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of HepG2 cells using CSB-PA011021LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) IFIT2 Polyclonal antibody

Uniprot No.

P09913

Target Names

IFIT2

Alternative Names

CIG-42 antibody; cig42 antibody; G10P2 antibody; GARG 39 antibody; Glucocorticoid attenuated response gene 39 antibody; IFI 54 antibody; IFI 54K antibody; IFI-54K antibody; IFI54 antibody; IFIT 2 antibody; IFIT-2 antibody; IFIT2 antibody; IFIT2_HUMAN antibody; Interferon induced 54 kDa protein antibody; Interferon induced protein 54 antibody; Interferon induced protein with tetratricopeptide repeats 2 antibody; Interferon, alpha inducible protein (MW 54kD) antibody; Interferon-induced 54 kDa protein antibody; Interferon-induced protein with tetratricopeptide repeats 2 antibody; ISG 54 K antibody; ISG 54K antibody; ISG-54 K antibody; ISG54 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Interferon-induced protein with tetratricopeptide repeats 2 protein (130-265AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The IFIT2 Antibody (Product code: CSB-PA011021LA01HU) is Non-conjugated. For IFIT2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA011021LB01HU	IFIT2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA011021LC01HU	IFIT2 Antibody, FITC conjugated
Biotin	CSB-PA011021LD01HU	IFIT2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.

Gene References into Functions

The results of the present study revealed that the inhibition of proteasome activity blocked the degradation of IFIT2 and promoted the aggregation of IFIT2 in the centrosome, which in turn induced cell apoptosis. In short, IFIT2 may be a potential target for cancer therapeutics. PMID: 28367102
Mechanistic investigations reveal that AJUBA specifically binds the FERM domain of JAK1 to dissociate JAK1 from the IFNgamma recepter, resulting in an inhibition of STAT1 phosporylation and concomitantly its nuclear translocation. Clinically, the level of AJUBA in CRC specimens is negatively correlated with the levels of IFIT2 and pSTAT1 PMID: 27893714
LINC00161 is an essential regulator in cisplatin-induced apoptosis, and the LINC00161-miR-645-IFIT2 signalling axis plays an important role in reducing osteosarcoma chemoresistance. PMID: 27609068
there is a positive feedback loop between phosphorylated STAT1 and ISG56, ISG54 or ISG60. PMID: 26423178
Data show that interferon-induced proteins with tetratricopeptide repeats 1 and 2 (IFIT1 and IFIT2) contribute to the regulation of hepatitis B virus (HBV) replication, likely at both transcriptional and posttranscriptional steps. PMID: 23867918
Data shows that IFIT2 expression is downregulated in adenocarcinoma of gastric esophageal junction cells; its ectopic expression leads to cell apoptosis, suggesting its role as a tumor suppressor. PMID: 25174799
IFIT2, a protein responsible for interferon stimulation, may prevent oral squamous cell carcinoma (OSCC) metastasis and serve as a valuable prognostic marker. PMID: 22986528
ISG54 binds specifically to some RNAs, such as adenylate uridylate (AU)-rich RNAs, with or without 5' triphosphorylation. PMID: 22825553
Inhibitor of kappaB kinase epsilon (IKK(epsilon)), STAT1, and IFIT2 proteins define novel innate immune effector pathway against West Nile virus infection. PMID: 22065572
The expression of OASL and IFIT2 was significantly higher in SLE patients than in controls. PMID: 20506645
role of ISG54 in the induction of apoptosis via a mitochondrial pathway shedding new light on the mechanism by which IFN elicits anti-viral and anti-cancer effects PMID: 21190939
The expression of interferon-induced genes IFI-54K and IFI-56K in the infected cells was found to increase 50-100-fold PMID: 12708317
We observed that, although double-stranded RNA or Sendai virus infection induced the two genes with similar kinetics, their induction kinetics in response to interferon-beta were quite different. PMID: 16973618
IFN-induced protein with tetratricopeptide repeats 2 inhibits migration activity and increases survival of oral squamous cell carcinoma. PMID: 18819931
IFIT2/GARG39 was identified as a microtubule-associated protein enriched in the mitotic spindle of mouse NIH3T3 and B16F10 melanoma cells. PMID: 17030862

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Subcellular Location

Cytoplasm. Endoplasmic reticulum.

Protein Families

IFIT family

Database Links

HGNC: 5409

OMIM: 147040

KEGG: hsa:3433

STRING: 9606.ENSP00000360891

UniGene: Hs.437609

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