INTU Antibody

Code CSB-PA891544LA01HU
Size US$166
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Image
  • IHC image of CSB-PA891544LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA891544LA01HU diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA891544LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) INTU Polyclonal antibody
Uniprot No.
Target Names
INTU
Alternative Names
Homolog of inturned (X. tropicalis) antibody; INT antibody; intu antibody; Inturned planar cell polarity effector homolog antibody; KIAA1284 antibody; PDZ domain containing 6 antibody; PDZ domain-containing protein 6 antibody; PDZD 6 antibody; PDZD6_HUMAN antibody; PDZK6 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein inturned protein (6-139AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The INTU Antibody (Product code: CSB-PA891544LA01HU) is Non-conjugated. For INTU Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA891544LB01HU INTU Antibody, HRP conjugated ELISA
FITC CSB-PA891544LC01HU INTU Antibody, FITC conjugated
Biotin CSB-PA891544LD01HU INTU Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Plays a key role in ciliogenesis and embryonic development. Regulator of cilia formation by controlling the organization of the apical actin cytoskeleton and the positioning of the basal bodies at the apical cell surface, which in turn is essential for the normal orientation of elongating ciliary microtubules. Plays a key role in definition of cell polarity via its role in ciliogenesis but not via conversion extension. Has an indirect effect on hedgehog signaling. Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies.
Subcellular Location
Cytoplasm. Cell surface. Cytoplasm, cytoskeleton, cilium basal body.
Protein Families
Inturned family
Database Links

HGNC: 29239

OMIM: 610621

KEGG: hsa:27152

STRING: 9606.ENSP00000334003

UniGene: Hs.391481

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