LSM4 Antibody

Code CSB-PA013207HA01HU
Size US$166
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  • Western blot
    All lanes: LSM4 antibody at 2µg/ml
    Lane 1: A549 whole cell lysate
    Lane 2: K562 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa
  • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA013207HA01HU at dilution of 1:200
  • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA013207HA01HU at dilution of 1:200
  • Immunofluorescent analysis of Hela cells using CSB-PA013207HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • Immunofluorescent analysis of HepG2 cells using CSB-PA013207HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) LSM4 Polyclonal antibody
Uniprot No.
Target Names
LSM4
Alternative Names
LSM4U6 snRNA-associated Sm-like protein LSm4 antibody; Glycine-rich protein antibody; GRP antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human U6 snRNA-associated Sm-like protein LSm4 protein (1-139AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The LSM4 Antibody (Product code: CSB-PA013207HA01HU) is Non-conjugated. For LSM4 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA013207HB01HU LSM4 Antibody, HRP conjugated ELISA
FITC CSB-PA013207HC01HU LSM4 Antibody, FITC conjugated
Biotin CSB-PA013207HD01HU LSM4 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.
Gene References into Functions
  1. Results show that the RGG domain of human Lsm4 stimulates processing bodies formation. Also, a novel interaction of the Lsm4 RGG domain with HAT1 and RBBP7 was discovered, leading to the possibility of a posttranslational modifications network involved in mRNP regulation. PMID: 27247266
  2. C-terminal extension of Lsm4 interacts directly with the histone mRNP, contacting both SLBP and 3'hExo. PMID: 24255165
  3. Data show that ICln159, a truncated ICln mutant, belongs to the pleckstrin homology (PH) domain family of proteins and interacts with LSm4, a protein involved in splicing and mRNA degradation. PMID: 15905169
  4. Gene induces transformed phenotype when overexpressed in a cancer breast cell line. PMID: 17178857
  5. LSm4 not only acts in RNA processing, but also as a co-factor in cell volume regulation. PMID: 19088440

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Subcellular Location
Nucleus.
Protein Families
SnRNP Sm proteins family
Database Links

HGNC: 17259

OMIM: 607284

KEGG: hsa:25804

STRING: 9606.ENSP00000469468

UniGene: Hs.515255

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