MAPKAPK3 Antibody

Code CSB-PA247267
Size US$297
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  • Western blot analysis of extracts from COLO205 cells, using MAPK3 antibody.
  • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using MAPK3 antibody.
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MAPKAPK3 Polyclonal antibody
Uniprot No.
Target Names
MAPKAPK3
Alternative Names
3pK antibody; anti MAPKAPK3 antibody; Chromosome 3p kinase antibody; EC 2.7.11.1 antibody; MAP kinase-activated protein kinase 3 antibody; MAPK activated protein kinase 3 antibody; MAPK-activated protein kinase 3 antibody; MAPK3_HUMAN antibody; MAPKAP antibody; MAPKAP kinase 3 antibody; MAPKAP-K3 antibody; MAPKAP3 antibody; MAPKAPK 3 antibody; MAPKAPK-3 antibody; Mapkapk3 antibody; MAPKAPK3 antibody antibody; Mitogen activated protein kinase activated protein kinase 3 antibody; Mitogen activated protein kinase activated protein kinase antibody; MK-3 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthesized peptide derived from internal of Human MAPK3.
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:3000
IHC 1:50-1:100
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
Gene References into Functions
  1. The natural history of MCRPE is in relation to the role of MAPKAPK3 in BM modeling, vascular endothelial growth factor activity, retinal pigment epithelial responses PMID: 27474146
  2. A dominant mutation in MAPKAPK3 causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium. PMID: 26744326
  3. MK3 modulation affects BMI1-dependent and independent cell cycle check-points PMID: 25853770
  4. findings reveal MK2/MK3 as crucial stress-responsive kinases that promote autophagy through Beclin 1 S90 phosphorylation PMID: 25693418
  5. Hepatitis C virus core protein interacted with MAPKAPK3 through amino acid residues 41 to 75 of core and the N-terminal half of kinase domain of MAPKAPK3. PMID: 23487458
  6. Identified CREB activators MAPKAPK3 and FHL5 as mediators of intimal hyperplasia in vein graft samples. PMID: 23127979
  7. A high-resolution (1.9 A) crystal structure of the highly homologous MK3 in complex with a pharmaceutical lead compound is presented. PMID: 19937655
  8. In the preconditioned heart, genes for MAPKAP kinase 3 were up-regulated. PMID: 11975836
  9. 3pK is transported to the cytoplasm upon both stress and mitogenic stimulation. While kinetics of nuclear export are similar in both situations, the activation pattern differs substantially. PMID: 15302577

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Involvement in disease
Macular dystrophy, patterned, 3 (MDPT3)
Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family
Tissue Specificity
Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain. Expressed in the retinal pigment epithelium.
Database Links

HGNC: 6888

OMIM: 602130

KEGG: hsa:7867

STRING: 9606.ENSP00000350639

UniGene: Hs.234521

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