MASTL Antibody

Datasheet

Code	CSB-PA846629LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate All lanes: MASTL antibody at 6.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 93 kDa Observed band size: 98 kDa IHC image of CSB-PA846629LA01HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA846629LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MASTL Polyclonal antibody

Uniprot No.

Q96GX5

Target Names

MASTL

Alternative Names

2700091H24Rik antibody; C88295 antibody; FLJ14813 antibody; GREATWALL antibody; Greatwall protein kinase antibody; GW antibody; GWL antibody; GWL_HUMAN antibody; hGWL antibody; MAST-L antibody; Mastl antibody; MGC117975 antibody; Microtubule associated serine/threonine kinase like antibody; Microtubule-associated serine/threonine-protein kinase-like antibody; RP11 85G18.2 antibody; Serine/threonine-protein kinase greatwall antibody; THC2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Serine/threonine-protein kinase greatwall protein (612-740AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MASTL Antibody (Product code: CSB-PA846629LA01HU) is Non-conjugated. For MASTL Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA846629LB01HU	MASTL Antibody, HRP conjugated	ELISA
FITC	CSB-PA846629LC01HU	MASTL Antibody, FITC conjugated
Biotin	CSB-PA846629LD01HU	MASTL Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.

Gene References into Functions

Using mathematical modelling, this paper confirms that deactivation of MASTL is essential for mitotic exit. PMID: 26872783
these results established that precise control of MASTL is essential to couple DNA damage to mitosis through the rate of mitotic entry and APC/C activation. PMID: 26923777
Thus, GWL is a human oncoprotein that promotes the hyperactivation of AKT via the degradation of its phosphatase, PHLPP, in human malignancies. PMID: 26613407
Thus, Fcp1 coordinates Cdk1 and Gwl inactivation to derepress PP2A-B55, generating a dephosphorylation switch that drives mitosis progression. PMID: 26653855
Boolean modeling identifies Greatwall/MASTL as an important regulator in the AURKA network of neuroblastoma. PMID: 26616283
Data show that siRNA knockdown of Forkhead box M1 (FOXM1) or microtubule-associated serine/threonine kinase-like (MASTL) induces radiosensitivity in non-small cell lung cancer (NSCLC). PMID: 25808837
Mastl upregulation is involved in cancer progression and tumor recurrence after initial cancer therapy PMID: 25373736
data demonstrate that GWL acts in a pathway with PP2A which is essential for prophase I exit and metaphase I microtubule assembly in mouse oocytes. PMID: 25472593
Taken together our results suggest a hierarchy of phosphatases coordinating Greatwall, Ensa/ARPP19 and Cdk substrate dephosphorylation during mitotic exit. PMID: 24391510
Studies indicate that mutations in three different genes within the THC2 locus have been associated with congenital thrombocytopenia, including a mutation in MASTL. PMID: 22102272
results identify Gwl as a member of the AGC family of kinases that appears to be regulated by unique mechanisms and that differs from the other members of this family PMID: 21444715
MASTL enhances cyclin B1-Cdk1-dependent mitotic phosphorylation events, directing mitotic entry, anaphase and cytokinesis in human cells. PMID: 20818157
A novel missense mutation in the human gene FLJ14813 is associated with autosomal dominant thrombocytopenia PMID: 12890928
A paper that narrows the identity of the gene for autosomal dominant thrombocytopenia (THC2) to FLJ14813. The mutation is present in all affected people across three generations while is absent in unaffected family members & 94 random blood donors. PMID: 12890928

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Involvement in disease

Defects in MASTL may play a role in the pathogenesis of thrombocytopenia, a disorder defined by reduced number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cleavage furrow.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family

Database Links

HGNC: 19042

OMIM: 608221

KEGG: hsa:84930

STRING: 9606.ENSP00000365107

UniGene: Hs.276905

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