MCF2 Antibody

Code CSB-PA013579LA01HU
Size US$166
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Image
  • IHC image of CSB-PA013579LA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA013579LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA013579LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MCF2 Polyclonal antibody
Uniprot No.
Target Names
MCF2
Alternative Names
MCF2 antibody; DBLProto-oncogene DBL antibody; Proto-oncogene MCF-2) [Cleaved into: MCF2-transforming protein; DBL-transforming protein] antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Proto-oncogene DBL protein (705-925AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MCF2 Antibody (Product code: CSB-PA013579LA01HU) is Non-conjugated. For MCF2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA013579LB01HU MCF2 Antibody, HRP conjugated ELISA
FITC CSB-PA013579LC01HU MCF2 Antibody, FITC conjugated
Biotin CSB-PA013579LD01HU MCF2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
Gene References into Functions
  1. Results support a role for Dbl oncogene in mammary epithelial cell differentiation and transformation and suggest the relevance of GEF deregulation in tumor onset and progression. PMID: 25723869
  2. Phosphoinositide 3-kinase C2beta regulates RhoA and the actin cytoskeleton through an interaction with Dbl PMID: 22984590
  3. Dbl is regulated by hamartin through association with ezrin. PMID: 21712385
  4. proto-oncogene dbl is not a major target for sporadic testicular germ cell tumors PMID: 19373475
  5. data describe 4 splicing variants of the human DBL (MCF-2) proto-oncogene that show different tissue distributions and guanine nucleotide exchange factor specificities PMID: 12445822
  6. provides fundamental data on the structure of Mcf-2, which regulates a variety of cellular signaling pathways PMID: 13679059
  7. These data suggest a mechanism whereby precise spatial guanine nucleotide exchange of Cdc42 by Dbl is dependent on functional ERM proteins and is important for directional cell migration. PMID: 17538024
  8. Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain PMID: 17606614
  9. Nm23-H1 can negatively regulate cell migration and tumor metastasis by modulating the activity of Cdc42 and possibly other Rho family members through interaction with Dbl-1 PMID: 18728402

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Involvement in disease
MCF2 and DBL represent two activated versions of the same proto-oncogene.
Subcellular Location
Cytoplasm.; [Isoform 1]: Membrane.; [Isoform 3]: Membrane. Note=Colocalizes with CDC42 to plasma membrane.
Protein Families
MCF2 family
Tissue Specificity
Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, int
Database Links

HGNC: 6940

OMIM: 311030

KEGG: hsa:4168

STRING: 9606.ENSP00000430276

UniGene: Hs.387262

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