MCHR2 Antibody

Datasheet

Code	CSB-PA013585LA01HU
Size	US$166
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Image	IHC image of CSB-PA013585LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA013585LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MCHR2 Polyclonal antibody

Uniprot No.

Q969V1

Target Names

MCHR2

Alternative Names

MCHR2; GPR145; SLT; Melanin-concentrating hormone receptor 2; MCH receptor 2; MCH-R2; MCHR-2; G-protein coupled receptor 145; GPRv17; MCH-2R; MCH2; MCH2R

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Melanin-concentrating hormone receptor 2 protein (1-39AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MCHR2 Antibody (Product code: CSB-PA013585LA01HU) is Non-conjugated. For MCHR2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA013585LB01HU	MCHR2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA013585LC01HU	MCHR2 Antibody, FITC conjugated
Biotin	CSB-PA013585LD01HU	MCHR2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Receptor for melanin-concentrating hormone, coupled to G proteins that activate phosphoinositide hydrolysis.

Gene References into Functions

evidence for the involvement of duplications in MCHR2 in alopecia areata pathogenesis in a Central European cohort PMID: 27306922
This study provides new insights on the possible influence of MCHR2 and/or MCHR2-AS1 on obesity in psychiatric patients and on the pathophysiology of atypical depression. PMID: 26461262
Discovery and characterization of a potent and selective antagonist of melanin-concentrating hormone receptor 2 PMID: 22123324
construction of the CHO cell line and the research of MCHR2 molecular characteristics have established a good experimental basis for the further research about the function of MCHR2 gene PMID: 20099459
MCH1 and MCH2 receptors were investigated and are different from those in SVK14 cells. PMID: 12127971
results of molecular simulations of MCHR2 in the free and hormone-bound forms provide suggestions on the amino acids responsible for the transfer of the structural changes from the agonist binding site to the G-protein coupling domains PMID: 15229878
MCHR2 is not a major contributor to polygenic obesity PMID: 17698913
MCHR2 positively mediates the regulation of melanin-concentrating hormone during preadipocyte differentiation and is involved in energy balance regulation without affecting preadipocyte proliferation. PMID: 19683862
The 10-amino-acid cyclic core of the hMCH neuropeptide with the Arg attached to the N-terminus of the disulfide ring is sufficient for full activation of melanin-concentrating hormone receptor 2. PMID: 11478907

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

Specifically expressed in the brain, with highest levels in cerebral cortex, hippocampus and amygdala. No expression detected in the cerebellum, thalamus or hypothalamus.

Database Links

HGNC: 20867

OMIM: 606111

KEGG: hsa:84539

STRING: 9606.ENSP00000281806

UniGene: Hs.591342

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

G protein coupled receptor signaling pathway Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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