MED27 Antibody

Code CSB-PA764716LA01HU
Size US$166
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Image
  • Western blot
    All lanes: MED27 antibody at 0.5µg/ml + A431 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 36, 32, 15 kDa
    Observed band size: 36 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MED27 Polyclonal antibody
Uniprot No.
Target Names
MED27
Alternative Names
Cofactor required for Sp1 transcriptional activation subunit 8 (34kD) antibody; Cofactor required for Sp1 transcriptional activation subunit 8 34kDa antibody; Cofactor required for Sp1 transcriptional activation subunit 8 antibody; CRAP 34 antibody; CRAP34 antibody; CRSP 34 antibody; CRSP 8 antibody; CRSP complex subunit 8 antibody; CRSP34 antibody; CRSP8 antibody; MED 27 antibody; Med27 antibody; MED27_HUMAN antibody; Mediator complex subunit 27 antibody; Mediator of RNA polymerase II transcription subunit 27 antibody; MGC11274 antibody; P37 TRAP/SMCC/PC2 subunit antibody; Transcriptional coactivator CRSP34 antibody; TRAP 37 antibody; TRAP37 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Mediator of RNA polymerase II transcription subunit 27 protein (39-108AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MED27 Antibody (Product code: CSB-PA764716LA01HU) is Non-conjugated. For MED27 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA764716LB01HU MED27 Antibody, HRP conjugated ELISA
FITC CSB-PA764716LC01HU MED27 Antibody, FITC conjugated
Biotin CSB-PA764716LD01HU MED27 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Gene References into Functions
  1. The findings of the present study may aid in the clarification of the function of miR18a, particularly as regards its role in the regulation of Osteosarcoma (OS)cell apoptosis, and indicate that MED27 may be a potential novel therapeutic target in the treatment of OS. PMID: 29693135
  2. MED27 promotes melanoma growth by targeting AKT/MAPK and NF-kappaB/iNOS signaling pathways. PMID: 26797421
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 27 family
Database Links

HGNC: 2377

OMIM: 605044

KEGG: hsa:9442

STRING: 9606.ENSP00000292035

UniGene: Hs.374262

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