MID2 Antibody

Datasheet

Code	CSB-PA892324ESR1HU
Size	US$166
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Image	Western blot All lanes: MID2 antibody at 3.32 μg/ml + Mouse small intestine tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 84, 80 kDa Observed band size: 84, 30 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA892324ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA892324ESR1HU at dilution of 1:100 Immunofluorescent analysis of MCF-7 cells using CSB-PA892324ESR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MID2 Polyclonal antibody

Uniprot No.

Q9UJV3

Target Names

MID2

Alternative Names

FLJ37715 antibody; FLJ41813 antibody; FXY2 antibody; MID2 antibody; Midin 2 antibody; Midin-2 antibody; Midline 2 antibody; Midline defect 2 antibody; Midline-2 antibody; probable E3 ubiquitin protein ligase MID2 antibody; Probable E3 ubiquitin-protein ligase MID2 antibody; RING finger protein 60 antibody; Ring finger protein protein 60 antibody; RNF60 antibody; TRIM1 antibody; TRIM1_HUMAN antibody; tripartite motif containing protein 1 antibody; Tripartite motif protein 1 antibody; Tripartite motif-containing protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Probable E3 ubiquitin-protein ligase MID2 protein (506-735AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May play a role in microtubule stabilization.

Gene References into Functions

overexpressed in advanced breast cancer and high overexpression is prognostic factor for poor overall survival PMID: 26791755
Mid2 regulates cell division through the ubiquitination of astrin on K409, which is critical for its degradation and proper cytokinesis. PMID: 26748699
A novel missense mutation (c.1040G>A, p.Arg347Gln) was reported in MID2, which encodes ubiquitin ligase E3, as the likely cause of X-linked mental retardation in a large kindred. PMID: 24115387
MID2 coiled-coil motifs mediate both homo- and heterodimerization, a prerequisite for association of the MID-alpha 4 complex with microtubules. PMID: 11806752
MID2 is a candidate gene for FG syndrome. PMID: 16283679

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Involvement in disease

Mental retardation, X-linked 101 (MRX101)

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.

Protein Families

TRIM/RBCC family

Tissue Specificity

Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.

Database Links

HGNC: 7096

OMIM: 300204

KEGG: hsa:11043

STRING: 9606.ENSP00000262843

UniGene: Hs.12256

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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