Recombinant Human Probable E3 ubiquitin-protein ligase MID2 (MID2)

Code CSB-YP892324HU
MSDS
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Source Yeast
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Code CSB-EP892324HU
MSDS
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Source E.coli
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Code CSB-EP892324HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892324HU
MSDS
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Source Baculovirus
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Code CSB-MP892324HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MID2
Uniprot No.
Alternative Names
FLJ37715; FLJ41813; FXY2; MID2; Midin 2; Midin-2; Midline 2; Midline defect 2; Midline-2; probable E3 ubiquitin protein ligase MID2; Probable E3 ubiquitin-protein ligase MID2; RING finger protein 60; Ring finger protein protein 60; RNF60; TRIM1; TRIM1_HUMAN; tripartite motif containing protein 1; Tripartite motif protein 1; Tripartite motif-containing protein 1
Species
Homo sapiens (Human)
Expression Region
1-735
Target Protein Sequence
MGESPASVVL NASGGLFSLK METLESELTC PICLELFEDP LLLPCAHSLC FSCAHRILVS SCSSGESIEP ITAFQCPTCR YVISLNHRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSES RRERTYRPTT AMSSERIACQ FCEQDPPRDA VKTCITCEVS YCDRCLRATH PNKKPFTSHR LVEPVPDTHL RGITCLDHEN EKVNMYCVSD DQLICALCKL VGRHRDHQVA SLNDRFEKLK QTLEMNLTNL VKRNSELENQ MAKLIQICQQ VEVNTAMHEA KLMEECDELV EIIQQRKQMI AVKIKETKVM KLRKLAQQVA NCRQCLERST VLINQAEHIL KENDQARFLQ SAKNIAERVA MATASSQVLI PDINFNDAFE NFALDFSREK KLLEGLDYLT APNPPSIREE LCTASHDTIT VHWISDDEFS ISSYELQYTI FTGQANFISK SWCSWGLWPE IRKCKEAVSC SRLAGAPRGL YNSVDSWMIV PNIKQNHYTV HGLQSGTRYI FIVKAINQAG SRNSEPTRLK TNSQPFKLDP KMTHKKLKIS NDGLQMEKDE SSLKKSHTPE RFSGTGCYGA AGNIFIDSGC HYWEVVMGSS TWYAIGIAYK SAPKNEWIGK NASSWVFSRC NSNFVVRHNN KEMLVDVPPH LKRLGVLLDY DNNMLSFYDP ANSLHLHTFD VTFILPVCPT FTIWNKSLMI LSGLPAPDFI DYPERQECNC RPQESPYVSG MKTCH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in microtubule stabilization.
Gene References into Functions
  1. overexpressed in advanced breast cancer and high overexpression is prognostic factor for poor overall survival PMID: 26791755
  2. Mid2 regulates cell division through the ubiquitination of astrin on K409, which is critical for its degradation and proper cytokinesis. PMID: 26748699
  3. A novel missense mutation (c.1040G>A, p.Arg347Gln) was reported in MID2, which encodes ubiquitin ligase E3, as the likely cause of X-linked mental retardation in a large kindred. PMID: 24115387
  4. MID2 coiled-coil motifs mediate both homo- and heterodimerization, a prerequisite for association of the MID-alpha 4 complex with microtubules. PMID: 11806752
  5. MID2 is a candidate gene for FG syndrome. PMID: 16283679

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Involvement in disease
Mental retardation, X-linked 101 (MRX101)
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Protein Families
TRIM/RBCC family
Tissue Specificity
Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
Database Links

HGNC: 7096

OMIM: 300204

KEGG: hsa:11043

STRING: 9606.ENSP00000262843

UniGene: Hs.12256

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