MIS12 Antibody

Datasheet

Code	CSB-PA875639LA01HU
Size	US$166
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Image	Western blot All lanes: MIS12 antibody at 2μg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA875639LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA875639LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MIS12 Polyclonal antibody

Uniprot No.

Q9H081

Target Names

MIS12

Alternative Names

MIS12 antibody; Protein MIS12 homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein MIS12 homolog protein (1-305AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MIS12 Antibody (Product code: CSB-PA875639LA01HU) is Non-conjugated. For MIS12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA875639LB01HU	MIS12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA875639LC01HU	MIS12 Antibody, FITC conjugated
Biotin	CSB-PA875639LD01HU	MIS12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.

Gene References into Functions

Whereas CENP-C recruits a single MIS12:NDC80 complex, the authors show here that CENP-T binds one MIS12:NDC80 and two NDC80 complexes upon phosphorylation by the mitotic CDK1:Cyclin B complex at three distinct CENP-T sites. PMID: 28012276
RWD-containing proteins interact with the Mis12 complex to shape KMN network topology, as revealed by structural analysis PMID: 24530301
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore. PMID: 21353556
These findings support a novel role for Hsp90-Sgt1 chaperones in ensuring the fidelity of Mis12 multiprotein complex assembly. PMID: 20404110
hMIS12 has a role in mitosis and localizes at kinetochores PMID: 12515822
These results indicate that a four-subunit Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. PMID: 16585270

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Subcellular Location

Chromosome, centromere, kinetochore.

Protein Families

Mis12 family

Database Links

HGNC: 24967

OMIM: 609178

KEGG: hsa:79003

STRING: 9606.ENSP00000370557

UniGene: Hs.267194

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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